整合素相关蛋白 (IAP/CD47) 激动剂肽 4N1K (KRFYVVMWKK)，源自血小板反应蛋白-1 (TS1) 的细胞结合域。该肽已被证明可以刺激依赖于整联蛋白的细胞功能，包括血小板聚集。
编号：190917
CAS号：178921-95-4
单字母：H2N-KRFYVVMWKK-OH
| 编号: | 190917 |
| 中文名称: | 血小板反应蛋白-I（TS-I）细胞结合结构域：[Lys1015, 1024]-Thrombospondin-1(1015-1024), human |
| 英文名: | [Lys1015, 1024]-Thrombospondin-1(1015-1024), human |
| CAS号: | 178921-95-4 |
| 单字母: | H2N-KRFYVVMWKK-OH |
| 三字母: | H2N N端氨基 -Lys赖氨酸 -Arg精氨酸 -Phe苯丙氨酸 -Tyr酪氨酸 -Val缬氨酸 -Val缬氨酸 -Met甲硫氨酸 -Trp色氨酸 -Lys赖氨酸 -Lys赖氨酸 -OHC端羧基 |
| 氨基酸个数: | 10 |
| 分子式: | C68H105N17O12S1 |
| 平均分子量: | 1384.73 |
| 精确分子量: | 1383.78 |
| 等电点(PI): | - |
| pH=7.0时的净电荷数: | 5.97 |
| 平均亲水性: | -0.16 |
| 疏水性值: | -0.49 |
| 外观与性状: | 白色粉末状固体 |
| 消光系数: | 6990 |
| 来源: | 人工化学合成，仅限科学研究使用，不得用于人体。 |
| 纯度: | 95%、98% |
| 盐体系: | 可选TFA、HAc、HCl或其它 |
| 生成周期: | 2-3周 |
| 储存条件: | 负80℃至负20℃ |
| 标签: | 激动剂多肽(Agonist Peptide) 血小板反应素 结构域Domain相关肽 细胞粘附肽(Cell Adhesion Peptides) |
整合素相关蛋白 (IAP/CD47) 激动剂肽 4N1K (KRFYVVMWKK)，源自血小板反应蛋白-1 (TS1) 的细胞结合域。该肽已被证明可以刺激依赖于整联蛋白的细胞功能，包括血小板聚集。
Integrin-associated protein (IAP/CD47) agonist peptide, 4N1K (KRFYVVMWKK), derived from the cell binding domain of thrombospondin-1 (TS1). The peptide has been shown to stimulate integrin-dependent cell functions, including platelet aggregation.
Discovery
Synthetic cell adhesion peptides were first derived from laminin. The peptide PA22-2 (CSRARKQAASIKVAVSADR-NH2) derived from laminin was found to function as a cell adhesion molecule which was tested with mouse mast cells1.
Classification
Cell adhesion peptides for the most part are extracellular matrix proteins1.
Structural Characteristics
Numerous cell adhesion peptides have been synthesized to date. Some examples include: RU-1 (LNIVSVNGRHX), RX-1 (DNRIRLQAKXX), GD-1 (KATPMLKMRTSFHGCIK), GD-2 (KEGYKVRLDLNITLEFRTTSK), GD-3 (KNLEISRSTFDLLRNSYGVRK), GD-6 (KQNCLSSRASFRGCVRNLRLSR), HGD-6 (KQKCLRSQTSFRGCLRKLALIK), SGD-6 (CRNRGRCNSSLFQVRSRKLLSA), HSGD-6 (KQCLKSQRSFTRGLCRLKAKIL), AG-1 (KLLISRARKQÁASIK), F17 (LERKYENDQKYLEDKA) and KRGD (VEKRGDREEA). Peptides that are linear and cyclic in nature have been synthesized2.
Mode of action
Cell adhesion peptides can bind to the cell membrane and trigger adhesion of cells3.
Functions
Cell adhesion peptides have known to increase signaling via receptors3. They have been shown to decrease tumor metastasis and growth in experimental animals3. These peptides also induce Ca2+ signaling and modulate platelet activity3.
References
1. Thompson HL, Burbelo PD,Yamada Y,Kleinman HK and Metcalfe DD (1991). Identification of an amino acid sequence in the laminin A chain mediating mast cell attachment and spreading, Immunology, 72, 144-149.
2. Gehlsen KR, Sriramarao P, Furcht LT and Skubitzt APN (1992). A Synthetic Peptide Derived from the Carboxy Terminus of the Laminin A Chain Represents a Binding Site for the a3ß1 Integrin, J Cell Biology,117 (2), 449-459.
3. Book: Proteins analysis and design, Angeletti RH, 222-226.
| DOI | 名称 | |
|---|---|---|
| 10.1182/blood-2002-11-3483 | Involvement of a CD47-dependent pathway in platelet adhesion on inflamed vascular endothelium under flow | 下载 |
| 10.1074/jbc.M302194200 | Thrombospondin-bound integrin-associated protein (CD47) physically and functionally modifies integrin alphaIIbbeta3 by its extracellular domain | 下载 |
| 10.1083/jcb.135.2.533 | Thrombospondin modulates alpha v beta 3 function through integrin-associated protein | 下载 |
| 10.1091/mbc.9.4.865 | The thrombospondin receptor CD47 (IAP) modulates and associates with alpha2 beta1 integrin in vascular smooth muscle cells | 下载 |
| 编号 | 名称 | CAS号 |
| 131140 | 血小板反应蛋白-I（TS-I）细胞结合结构域、Thrombospondin-1 (1016-1023) (human, bovine, mouse) | 149234-04-8 |
| H2N-RFYVVMWK-OH | ||
| 八肽 RFYVVMWK 构成了名为 C₄ 的 30 聚体肽中的活性序列，因为它对 TS1 CBD 的细胞附着活性至关重要。它支持 G361 黑色素瘤细胞的附着并抑制它们与 TS1 的 rec CBD 的粘附。此外，该肽似乎在所有 TS1 亚型中都高度保守，并且在层粘连蛋白的 F9 片段中也存在相关序列。4N1 已被用作 CD47 激动剂。 | ||
| 416519 | 血小板反应蛋白-I（TS-I）细胞结合结构域：Cys-[Lys1015, 1024]-Thrombospondin-1(1015-1024), human | |
| H2N-CKRFYVVMWKK-OH | ||
| 在整合素相关蛋白 (IAP/CD47) 激动剂肽 4N1K (KRFYVVMWKK)的N端添加Cys | ||
![178921-95-4,[Lys1015, 1024]-Thrombospondin-1(1015-1024), human,H2N-Lys-Arg-Phe-Tyr-Val-Val-Met-Trp-Lys-Lys-COOH,H2N-KRFYVVMWKK-OH,杭州专肽生物的产品](/static/ah/images/def.jpg)
