Synthetic N-procalcitonin (N-PCT) has been shown to stimulate proliferation of normal and neoplastic human osteoblasts at nanomolar concentrations. Tavares et al. have demonstrated that intracerebroventricular administration of N-PCT significantly decreas
编号:122434
CAS号:118277-01-3
单字母:H2N-APFRSALESSPADPATLSEDEARLLLAALVQDYVQMKASELEQEQEREGSSLDSPRS-OH
编号: | 122434 |
中文名称: | N-proCTAmino-terminalProcalcitonin |
英文名: | N-proCTAmino-terminalProcalcitonin |
英文同义词: | N-Procalcitonin (human), N-PCT (human), N-proCT Amino-terminal Procalcitonin |
CAS号: | 118277-01-3 |
单字母: | H2N-APFRSALESSPADPATLSEDEARLLLAALVQDYVQMKASELEQEQEREGSSLDSPRS-OH |
三字母: | H2N N端氨基 -Ala丙氨酸 -Pro脯氨酸 -Phe苯丙氨酸 -Arg精氨酸 -Ser丝氨酸 -Ala丙氨酸 -Leu亮氨酸 -Glu谷氨酸 -Ser丝氨酸 -Ser丝氨酸 -Pro脯氨酸 -Ala丙氨酸 -Asp天冬氨酸 -Pro脯氨酸 -Ala丙氨酸 -Thr苏氨酸 -Leu亮氨酸 -Ser丝氨酸 -Glu谷氨酸 -Asp天冬氨酸 -Glu谷氨酸 -Ala丙氨酸 -Arg精氨酸 -Leu亮氨酸 -Leu亮氨酸 -Leu亮氨酸 -Ala丙氨酸 -Ala丙氨酸 -Leu亮氨酸 -Val缬氨酸 -Gln谷氨酰胺 -Asp天冬氨酸 -Tyr酪氨酸 -Val缬氨酸 -Gln谷氨酰胺 -Met甲硫氨酸 -Lys赖氨酸 -Ala丙氨酸 -Ser丝氨酸 -Glu谷氨酸 -Leu亮氨酸 -Glu谷氨酸 -Gln谷氨酰胺 -Glu谷氨酸 -Gln谷氨酰胺 -Glu谷氨酸 -Arg精氨酸 -Glu谷氨酸 -Gly甘氨酸 -Ser丝氨酸 -Ser丝氨酸 -Leu亮氨酸 -Asp天冬氨酸 -Ser丝氨酸 -Pro脯氨酸 -Arg精氨酸 -Ser丝氨酸 -OHC端羧基 |
氨基酸个数: | 57 |
分子式: | C264H426N74O97S1 |
平均分子量: | 6220.71 |
精确分子量: | 6217.04 |
等电点(PI): | 4.19 |
pH=7.0时的净电荷数: | -5.01 |
平均亲水性: | 0.49038461538462 |
疏水性值: | -0.62 |
外观与性状: | 白色粉末状固体 |
消光系数: | 1490 |
来源: | 人工化学合成,仅限科学研究使用,不得用于人体。 |
纯度: | 95%、98% |
盐体系: | 可选TFA、HAc、HCl或其它 |
生成周期: | 2-3周 |
储存条件: | 负80℃至负20℃ |
标签: | 未分类肽 |
Synthetic N-procalcitonin (N-PCT) has been shown to stimulate proliferation of normal and neoplastic human osteoblasts at nanomolar concentrations. Tavares et al. have demonstrated that intracerebroventricular administration of N-PCT significantly decreased food intake and body weight gain for at least 48 h in conscious, freely moving, and unstressed rats fed ad libitum.
DOI | 名称 | |
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10.1359/jbmr.2002.17.10.1872 | Involvement of cyclic adenosine monophosphate-dependent protein kinase A and pertussis toxin-sensitive G proteins in the migratory response of human CD14+ mononuclear cells to katacalcin | 下载 |
10.1007/s00134-003-1759-2 | Calcitonin gene related peptide and N-procalcitonin modulate CD11b upregulation in lipopolysaccharide activated monocytes and neutrophils | 下载 |
10.1210/en.2006-0792 | N-procalcitonin: central effects on feeding and energy homeostasis in rats | 下载 |
10.1111/j.1365-2826.2012.02308.x | Central administration of aminoprocalcitonin inhibits food intake and stimulates the hypothalamic-pituitary-adrenal axis in rats via the corticotrophin-releasing factor system | 下载 |
10.1073/pnas.86.23.9519 | Procalcitonin's amino-terminal cleavage peptide is a bone-cell mitogen | 下载 |
多肽H2N-Ala-Pro-Phe-Arg-Ser-Ala-Leu-Glu-Ser-Ser-Pro-Ala-Asp-Pro-Ala-Thr-Leu-Ser-Glu-Asp-Glu-Ala-Arg-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln-Asp-Tyr-Val-Gln-Met-Lys-Ala-Ser-Glu-Leu-Glu-Gln-Glu-Gln-Glu-Arg-Glu-Gly-Ser-Ser-Leu-Asp-Ser-Pro-Arg-Ser-COOH的合成步骤:
1、合成CTC树脂:称取0.75g CTC Resin(如初始取代度约为0.92mmol/g)和0.83mmol Fmoc-Ser(tBu)-OH于反应器中,加入适量DCM溶解氨基酸(需要注意,此时CTC树脂体积会增大好几倍,避免DCM溶液过少),再加入2.07mmol DIPEA(Mw:129.1,d:0.740g/ml),反应2-3小时后,可不抽滤溶液,直接加入1ml的HPLC级甲醇,封端半小时。依次用DMF洗涤2次,甲醇洗涤1次,DCM洗涤一次,甲醇洗涤一次,DCM洗涤一次,DMF洗涤2次(这里使用甲醇和DCM交替洗涤,是为了更好地去除其他溶质,有利于后续反应)。得到 Fmoc-Ser(tBu)-CTC Resin。结构图如下:
2、脱Fmoc:加3倍树脂体积的20%Pip/DMF溶液,鼓氮气30分钟,然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Ser(tBu)-CTC Resin 。(此步骤脱除Fmoc基团,茚三酮检测为蓝色,Pip为哌啶)。结构图如下:
3、缩合:取2.07mmol Fmoc-Arg(Pbf)-OH 氨基酸,加入到上述树脂里,加适当DMF溶解氨基酸,再依次加入4.14mmol DIPEA,1.97mmol HBTU。反应30分钟后,取小样洗涤,茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。(洗涤树脂,去掉残留溶剂,为下一步反应做准备)。得到Fmoc-Arg(Pbf)-Ser(tBu)-CTC Resin。氨基酸:DIPEA:HBTU:树脂=3:6:2.85:1(摩尔比)。结构图如下:
4、依次循环步骤二、步骤三,依次得到
H2N-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Pro-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Pro-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Ser(tBu)-Pro-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Ser(tBu)-Pro-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Ser(tBu)-Ser(tBu)-Pro-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Ser(tBu)-Ser(tBu)-Pro-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Glu(OtBu)-Ser(tBu)-Ser(tBu)-Pro-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Glu(OtBu)-Ser(tBu)-Ser(tBu)-Pro-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Leu-Glu(OtBu)-Ser(tBu)-Ser(tBu)-Pro-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Leu-Glu(OtBu)-Ser(tBu)-Ser(tBu)-Pro-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Ala-Leu-Glu(OtBu)-Ser(tBu)-Ser(tBu)-Pro-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Ala-Leu-Glu(OtBu)-Ser(tBu)-Ser(tBu)-Pro-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ser(tBu)-Ser(tBu)-Pro-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ser(tBu)-Ser(tBu)-Pro-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Arg(Pbf)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ser(tBu)-Ser(tBu)-Pro-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Arg(Pbf)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ser(tBu)-Ser(tBu)-Pro-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Phe-Arg(Pbf)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ser(tBu)-Ser(tBu)-Pro-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Phe-Arg(Pbf)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ser(tBu)-Ser(tBu)-Pro-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Pro-Phe-Arg(Pbf)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ser(tBu)-Ser(tBu)-Pro-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
H2N-Pro-Phe-Arg(Pbf)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ser(tBu)-Ser(tBu)-Pro-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
Fmoc-Ala-Pro-Phe-Arg(Pbf)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ser(tBu)-Ser(tBu)-Pro-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin
以上中间结构,均可在专肽生物多肽计算器-多肽结构计算器中,一键画出。
最后再经过步骤二得到 H2N-Ala-Pro-Phe-Arg(Pbf)-Ser(tBu)-Ala-Leu-Glu(OtBu)-Ser(tBu)-Ser(tBu)-Pro-Ala-Asp(OtBu)-Pro-Ala-Thr(tBu)-Leu-Ser(tBu)-Glu(OtBu)-Asp(OtBu)-Glu(OtBu)-Ala-Arg(Pbf)-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln(Trt)-Asp(OtBu)-Tyr(tBu)-Val-Gln(Trt)-Met-Lys(Boc)-Ala-Ser(tBu)-Glu(OtBu)-Leu-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Glu(OtBu)-Gly-Ser(tBu)-Ser(tBu)-Leu-Asp(OtBu)-Ser(tBu)-Pro-Arg(Pbf)-Ser(tBu)-CTC Resin,结构如下:
5、切割:6倍树脂体积的切割液(或每1g树脂加8ml左右的切割液),摇床摇晃 2小时,过滤掉树脂,用冰无水乙醚沉淀滤液,并用冰无水乙醚洗涤沉淀物3次,最后将沉淀物放真空干燥釜中,常温干燥24小试,得到粗品H2N-Ala-Pro-Phe-Arg-Ser-Ala-Leu-Glu-Ser-Ser-Pro-Ala-Asp-Pro-Ala-Thr-Leu-Ser-Glu-Asp-Glu-Ala-Arg-Leu-Leu-Leu-Ala-Ala-Leu-Val-Gln-Asp-Tyr-Val-Gln-Met-Lys-Ala-Ser-Glu-Leu-Glu-Gln-Glu-Gln-Glu-Arg-Glu-Gly-Ser-Ser-Leu-Asp-Ser-Pro-Arg-Ser-COOH。结构图见产品结构图。
切割液选择:1)TFA:H2O=95%:5%
2)TFA:H2O:TIS=95%:2.5%:2.5%
3)三氟乙酸:茴香硫醚:1,2-乙二硫醇:苯酚:水=87.5%:5%:2.5%:2.5%:2.5%
(前两种适合没有容易氧化的氨基酸,例如Trp、Cys、Met。第三种适合几乎所有的序列。)
6、纯化冻干:使用液相色谱纯化,收集目标峰液体,进行冻干,获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度 是否目标纯度。
7、最后总结:
杭州专肽生物技术有限公司(ALLPEPTIDE https://www.allpeptide.com)主营定制多肽合成业务,提供各类长肽,短肽,环肽,提供各类修饰肽,如:荧光标记修饰(CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等),功能基团修饰肽(叠氮、炔基、DBCO、DOTA、NOTA等),同位素标记肽(N15、C13),订书肽(Stapled Peptide),脂肪酸修饰肽(Pal、Myr、Ste),磷酸化修饰肽(P-Ser、P-Thr、P-Tyr),环肽(酰胺键环肽、一对或者多对二硫键环),生物素标记肽,PEG修饰肽,甲基化修饰肽等。
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