Gap 26 是一种连接蛋白 (connexin) 模拟肽,由连接蛋白 connexin 43 (gap junction 阻滞剂)。
编号:115270
CAS号:197250-15-0
单字母:H2N-VCYDKSFPISHVR-OH
编号: | 115270 |
中文名称: | 间隙连接的阻断肽:GAP26 |
英文名: | GAP26 |
CAS号: | 197250-15-0 |
单字母: | H2N-VCYDKSFPISHVR-OH |
三字母: | H2N N端氨基 -Val缬氨酸 -Cys半胱氨酸 -Tyr酪氨酸 -Asp天冬氨酸 -Lys赖氨酸 -Ser丝氨酸 -Phe苯丙氨酸 -Pro脯氨酸 -Ile异亮氨酸 -Ser丝氨酸 -His组氨酸 -Val缬氨酸 -Arg精氨酸 -OHC端羧基 |
氨基酸个数: | 13 |
分子式: | C70H107N19O19S1 |
平均分子量: | 1550.78 |
精确分子量: | 1549.77 |
等电点(PI): | 11.71 |
pH=7.0时的净电荷数: | 4.18 |
平均亲水性: | -0.21666666666667 |
疏水性值: | -0.08 |
消光系数: | 1490 |
来源: | 人工化学合成,仅限科学研究使用,不得用于人体。 |
盐体系: | 可选TFA、HAc、HCl或其它 |
储存条件: | 负80℃至负20℃ |
标签: | 层粘连蛋白(Laminins) 阻断剂相关肽(Blocker Peptide) |
Gap 26 TFA 是一种连接蛋白 (connexin) 模拟肽,由连接蛋白 connexin 43 (gap junction 阻滞剂)第一个胞外环的第 63-75 残基组成,包含SHVR氨基酸基序。
Gap 26 TFA is a connexin mimetic peptide, composed of residue numbers 63-75 of the first extracellular loop of connexin 43 (gap junction blocker), containing the SHVR amino acid motif.
背景
Gap26 (Val-Cys-Tyr-Asp-Lys-Ser-Phe-Pro-Ile-Ser-His-Val-Arg)是一种连接蛋白模拟肽,对应于connexin 43的63-75 aa,是间隙连接的阻断肽。
连接蛋白或间隙连接是一个结构相关的转膜蛋白家族。间隙连接包含允许离子和小分子穿过相邻细胞的通道。钙和磷酸肌醇是可以穿过间隙连接通道的第二信使[1]。据报道,gap26可以衰减兔动脉平滑肌的节律收缩活动,IC50值为28.4 mM。Gap26也可以阻断IP3诱导的ATP和Ca2+穿过连接蛋白半通道,即六聚通道,与相邻细胞对接从而形成间隙连接的管道[2]。
参考文献:
1. Boitano, S. and H. Evans Am. J. Physiol. Lung Cell Mol. Physiol. 279, L623 (2000).
2. T. Desplantez, V. Verma, L. Leybaert, W.H. Evans, R. Weingart, Gap26, a connexin mimetic peptide, inhibits currents carried by connexin43 hemichannels and gap junction channels, Pharmacological Research, Volume 65, Issue 5, May 2012, Pages 546-552.
Laminins are the protein network that is the foundation for most cells and organs. They are large trimeric proteins, also in the family of glycoproteins that contain an alpha chain, a beta chain and a gamma chain which are found in five, three and three genetic variants. As a family of glycoproteins, laminins are an integral part of the structural scaffolding in almost every tissue of an organism. This makes the laminin a vital part of the maintenance and survival of the tissues. Consequently, defective laminins cause muscular dystrophy, junctional epidermolysis bullosa and defects of the kidney a defect.
Recently, publications have shown that laminins can be used to culture cells that are difficult to culture on other substrates. These trimeric glycoproteins form a similar structure to a cross, giving it a structure that has the ability to bind to other cell membrane and extracellular molecules. The three shorter arms of the laminin are particularly efficient at binding to other laminin molecules, which allows them to form larger sheets. The long arm is capable of binding to cells, which then helps anchor organized tissue cells to the membrane. These laminins are an important and biologically active part of the basal lamina, influenincing cell differentiation, migration and adhesion.
DOI | 名称 | |
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10.1016/s0143-4160(02)00180-x | Photoliberating inositol-1,4,5-trisphosphate triggers ATP release that is blocked by the connexin mimetic peptide gap 26 | 下载 |
10.1111/j.1469-7793.1997.099bi.x | Peptides homologous to extracellular loop motifs of connexin 43 reversibly abolish rhythmic contractile activity in rabbit arteries | 下载 |