A hydrophilic two-dimensional titanium-based metal-organic framework nanosheets for

specific enrichment of glycopeptides

Yang, SS (Yang, Shi-Shu)[ 1 ] ; Wang, C (Wang, Chen)[ 1 ] ; Yu, XZ (Yu, Xizhong)[ 2 ] ;

Shang, WB (Shang, Wenbin)[ 2 ] ; Chen, DDY (Chen, David Da Yong)[ 1,3 ]*; Gu, ZY (Gu, Zhi-

Yuan)[ 1 ]*（古志远）

[ 1 ]Nanjing Normal Univ, Jiangsu Key Lab Biofunct Mat, Jiangsu Collaborat Innovat Ctr

Biomed Funct Mat, Coll Chem & Mat Sci, Nanjing 210023, Peoples R China
[ 2 ]Nanjing Univ Chinese Med, Key Lab Metab Dis Chinese Med, Coll Clin Med 1, Nanjing

210023, Peoples R China
[ 3 ]Univ British Columbia, Dept Chem, Vancouver, BC V6T 1Z1, Canada

 

ANALYTICA CHIMICA ACTA，202007,1119,60-67

 

A hydrophilic two-dimensional titanium-based metal-organic framework (MOF) nanosheets were

prepared and applied to improve the glycopeptides enrichment efficiency. Seventeen N-linked

glycopeptides from horseradish peroxidase (HRP) tryptic digests were readily identified and

the results showed highly improved signal intensities. As the molar ratio of the mixture of

tryptic digests of HRP, glycoprotein (HRP), and nonglycoprotein (BSA) reached 1:800:800,

good selectivity of the Ti-MOF nanosheets were still clearly observed. As to human serum

samples, 41 glycoproteins and 66 glycopeptides corresponding to 68 N-glycosylation sites

were finally detected, which showed the good ability of the nanosheets material to enrich

N-linked glycopeptides from complex human serum samples. Moreover, the Ti-MOF nanosheets

exhibited unique stability and reusability even after being regenerated and reused for five

times. It also performed a high sensitivity (1 x 10(-10) M HRP), a good enrichment capacity

(250 mg/g) for glycopeptides enrichment and a high enrichment recovery of 78%, suggesting a

great application prospect for the enrichment of N-linked glycopeptides and the analysis

glycoproteomic research.

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https://www.sciencedirect.com/science/article/pii/S0003267020304839?via%3Dihub

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