Raman observation of a molecular signaling pathway of apoptotic cells induced by photothermal therapy
Xing, YF (Xing, Yingfang)[ 1 ] ; Cai, ZW (Cai, Zhewei)[ 4 ] ; Xu, MJ (Xu, Meijuan)[ 3 ] ; Ju, WZ (Ju, Wenzheng)[ 3 ] ; Luo, XJ (Luo, Xiaojun)[ 1 ] ; Hu, YJ (Hu, Yaojuan)[ 1 ] ; Liu, XY (Liu, Xiaoyan)[ 1 ] ; Kang, TL (Kang, Tuli)[ 1 ] ; Wu, P (Wu, Ping)[ 1 ]（吴萍）* ; Cai, CX (Cai, Chenxin)[ 1 ]（蔡称心）* ; Zhu, JJ (Zhu, Jun-Jie)[ 2 ]*

[ 1 ] Nanjing Normal Univ, Jiangsu Collaborat Innovat Ctr Biomed Funct Mat, Natl & Local Joint Engn Res Ctr Biomed Funct Mat, Coll Chem & Mat Sci,Jiangsu Key Lab New Power Bat, Nanjing 210097, Jiangsu, Peoples R China
[ 2 ] Nanjing Univ, State Key Lab Analyt Life Sci, Sch Chem & Chem Engn, Nanjing 210093, Jiangsu, Peoples R China
[ 3 ] Nanjing Univ Chinese Med, Affiliated Hosp, Key Lab Dept Clin Pharmacol, Nanjing, Jiangsu, Peoples R China
[ 4 ] Clarkson Univ, Dept Chem & Biomol Engn, Potsdam, NY 13699 USA

CHEMICAL SCIENCE,201912,10(47), 10900-10910

Plasmonic nanoparticle (NP)-mediated photothermal therapy (PPTT) has been explored as a minimally invasive approach to cancer therapy and has progressed from concept to the early stage of clinical trials. Better understanding of the cellular and molecular response to PPTT is crucial for improvement of therapy efficacy and advancement of clinical application. However, the molecular mechanism underlying PPTT-induced apoptosis is still unclear and under dispute. In this work, we used nuclear-targeting Au nanostars (Au NSs) as both a photothermal agent to specifically induce apoptosis in cancer cells and as a surface enhanced Raman spectroscopy (SERS) probe to monitor the time-dependent SERS spectra of MCF-7 cells which are undergoing apoptosis. Through SERS spectra and their synchronous and asynchronous SERS correlation maps, the occurrence and dynamics of a cascade of molecular events have been investigated, and a molecular signaling pathway of PPTT-induced apoptosis, including release of cytochrome c, protein degradation, and DNA fragmentation, was revealed, which was also demonstrated by metabolomics, agarose gel electrophoresis, and western blot analysis, respectively. These results indicated that PPTT-induced apoptosis undergoes an intrinsic mitochondria-mediated apoptosis pathway. Combined with western blot results, this intrinsic mitochondria-mediated apoptosis pathway was further demonstrated to be initiated by a BH3-only protein, BID. This work is beneficial for not only improving the fundamental understanding of the molecular mechanism of apoptosis induced by PPTT but also for guiding the modulation of PPTT to drive forward its clinical application.

文章链接：
https://pubs.rsc.org/en/content/articlelanding/2019/SC/C9SC04389F#!divAbstract

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