A dual-responsive biosensor for blood lead detection
Chen, H (Chen, Huan)[ 1 ] ; Shao, SB (Shao, Shuibin)[ 1 ] ; Yu, YQ (Yu, Yueqi)[ 1 ] ; Huang, YY (Huang, Yangyang)[ 1 ] ; Zhu, XT (Zhu, Xiaotan)[ 1 ] ; Zhang, SY (Zhang, Shiyan)[ 1 ] ; Fan, J (Fan, Jin)[ 2 ] ; Yin, GY (Yin, Guo Yong)[ 2 ] ; Chi, B (Chi, Bo)[ 3 ] ; Wan, MM (Wan, Mimi)[ 1 ]*（万密密）; Mao, C (Mao, Chun)[ 1 ]*（毛春）

[ 1 ] Nanjing Normal Univ, Sch Chem & Mat Sci, Natl & Local Joint Engn Res Ctr Biomed Funct Mat, Nanjing 210023, Jiangsu, Peoples R China
[ 2 ] Nanjing Med Univ, Affiliated Hosp 1, Dept Orthopaed, Nanjing 211166, Jiangsu, Peoples R China
[ 3 ] Nanjing Tech Univ, Coll Food Sci & Light Ind, State Key Lab Mat Oriented Chem Engn, Nanjing 211816, Peoples R China

ANALYTICA CHIMICA ACTA，202001,1093,131-141

Simple and accurate detection of trace heavy metals in blood is very important. A novel dual-responsive electrochemical/fluorescent biosensor based on magnetic hyperbranched polyamide with heparin modification (MHPAM-H) for blood lead detection has been successfully developed. Upon conjugated with blood lead ions, dual-biosensor could not only display electrochemical signal but also fluorescence signal owing to the enriched amino groups, cavity structure, and good fluorescence properties of HPAM. Blood biocompatibility, construction of the dual-responsive biosensor, electrochemical/fluorescent detection of lead ions in water phase and blood condition, selectivity and stability of the dual-responsive biosensor were investigated in detail. The proposed dual-responsive biosensor displays good linear relationship (1.5 pM-4.8 x 10(3) pM for electrochemical detection and 0.5 pM-4.8 x 10(3) pM for fluorescent detection) with low detection limit (4.4 pM for electrochemical detection and 1.0 pM for fluorescent detection) for blood lead, providing potential application for blood lead detection in the future.

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https://www.sciencedirect.com/science/article/pii/S0003267019311493?via%3Dihub

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