Cascade signal amplification sensing strategy for highly specific and sensitive detection of homologous microRNAs in different molecular subtypes of breast cancer
Qiao, L (Qiao, Ling)[ 1 ] ; Wu, CL (Wu, Chuanli)[ 1 ] ; Cai, ZW (Cai, Zhewei)[ 2 ] ; Wu, XC (Wu, Xingchen)[ 1 ] ; Wu, P (Wu, Ping)[ 1 ]*（吴萍）; Cai, CX (Cai, Chenxin)[ 1 ]*（蔡称心）

[ 1 ] Nanjing Normal Univ, Coll Chem & Mat Sci, Jiangsu Collaborat Innovat Ctr Biomed Funct Mat, Jiangsu Key Lab New Power Batteries, Nanjing 210097, Jiangsu, Peoples R China
[ 2 ] Clarkson Univ, Dept Chem & Biomol Engn, Potsdam, NY 13699 USA

ANALYTICA CHIMICA ACTA，202001,1093,86-92

Discriminative identification of homologous miRNAs in miRNA family with high specificity and sensitivity is crucial for accurate classification, diagnosis and prognosis of breast cancer. Herein, we report a reliable, sensitive, and selective assay by coupling fluorescence resonance energy transfer (FRET) with cascade signal amplification. The strategy is developed by designing two programmable DNA probes that can be triggered to shift from "off" to "on" state in a cascade hybridization reaction in the presence of target miRNA let-7a, leading to the generation of an amplified signal. The assay can detect concentrations as low as similar to 3.0 pM let-7a and discriminate let-7a from other highly homologous members in the let-7 miRNA family. Moreover, it can also be used to determine let-7a levels at single-cell resolution and evaluate the drug efficacy of let-7a expression among various molecular types of breast cancer cell lines. The advantage of this assay is a combined result of signal generation and amplification triggered by target miRNA, which can satisfy an assay of analogous miRNA in a downregulated manner with high specificity. It has promising potential as a selective assay for homologous miRNAs in precision medicine.

文章链接：
https://www.sciencedirect.com/science/article/pii/S0003267019311092?via%3Dihub

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