Arabinogalactan protein-rare earth element complexes activate plant endocytosis
Wang, LH (Wang, Lihong)[ 1,2 ] ; Cheng, MZ (Cheng, Mengzhu)[ 1 ] ; Yang, Q (Yang, Qing)[ 1 ] ; Li, JG (Li, Jigang)[ 3 ] ; Wang, X (Wang, Xiang)[ 1 ] ; Zhou, Q (Zhou, Qing)[ 2 ] ; Nagawa, S (Nagawa, Shingo)[ 4,5 ] ; Xia, BX (Xia, Binxin)[ 2 ] ; Xu, TD (Xu, Tongda)[ 4,5 ] ; Huang, RF (Huang, Rongfeng)[ 4,5 ] ; He, JF (He, Jingfang)[ 1 ] ; Li, CJ (Li, Changjiang)[ 3 ] ; Fu, Y (Fu, Ying)[ 3 ] ; Liu, Y (Liu, Ying)[ 1 ] ; Bao, JC (Bao, Jianchun)[ 1 ] ; Wei, HY (Wei, Haiyan)[ 1 ] ; Li, H (Li, Hui)[ 5,6 ] ; Tan, L (Tan, Li)[ 5 ] ; Gu, ZH (Gu, Zhenhong)[ 5 ] ; Xia, A (Xia, Ao)[ 1 ] ; Huang, XH (Huang, Xiaohua)[ 1 ]*（黄晓华） ; Yang, ZB (Yang, Zhenbiao)[ 7,8 ] ; Deng, XW (Deng, Xing Wang)[ 9,10 ]*

[ 1 ] Nanjing Normal Univ, Natl & Local Joint Engn Res Ctr Biomed Funct Mat, Jiangsu Collaborat Innovat Ctr Biomed Funct Mat, Sch Chem & Mat Sci, Nanjing 210023, Jiangsu, Peoples R China
[ 2 ] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
[ 3 ] China Agr Univ, China State Key Lab Plant Physiol & Biochem, Coll Biol Sci, Beijing 100193, Peoples R China
[ 4 ] Fujian Agr & Forestry Univ Univ California, Riverside Joint Ctr Hort Biol & Metabol, Haixia Inst Sci & Technol, Fujian Agr & Forestry Univ, Fuzhou 350002, Fujian, Peoples R China
[ 5 ] Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai Inst Biol Sci, Shanghai 201602, Peoples R China
[ 6 ] East China Normal Univ, Sch Life Sci, Shanghai 200241, Peoples R China
[ 7 ] Univ Calif Riverside, Ctr Plant Cell Biol, Inst Integrat Genome Biol, Riverside, CA 92521 USA
[ 8 ] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA
[ 9 ] Peking Univ, State Key Lab Prot & Plant Gene Res, Peking Tsinghua Ctr Life Sci, Sch Adv Agr Sci, Beijing 100871, Peoples R China

[ 10 ] Peking Univ, Sch Life Sci, Beijing 100871, Peoples R China

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA，201907,116(28),14349-14357

Endocytosis is essential to all eukaryotes, but how cargoes are selected for internalization remains poorly characterized. Extracellular cargoes are thought to be selected by transmembrane receptors that bind intracellular adaptors proteins to initiate endocytosis. Here, we report a mechanism for clathrin-mediated endocytosis (CME) of extracellular lanthanum [La(III)] cargoes, which requires extracellular arabinogalactan proteins (AGPs) that are anchored on the outer face of the plasma membrane. AGPs were colocalized with La(III) on the cell surface and in La(III)induced endocytic vesicles in Arabidopsis leaf cells. Superresolution imaging showed that La(III) triggered AGP movement across the plasma membrane. AGPs were then colocalized and physically associated with the it subunit of the intracellular adaptor protein 2 (AP2) complexes. The AGP-AP2 interaction was independent of CME, whereas AGP's internalization required CME and AP2. Moreover, we show that AGP-dependent endocytosis in the presence of La(III) also occurred in human cells. These findings indicate that extracellular AGPs act as conserved CME cargo receptors, thus challenging the current paradigm about endocytosis of extracellular cargoes.

文章链接：
https://www.pnas.org/content/116/28/14349

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