Flagelin 22 (Flagellin 22) 是细菌鞭毛素的一个片段,在植物和藻类中都是有效的诱导剂。
编号:151113
CAS号:304642-91-9
单字母:H2N-QRLSTGSRINSAKDDAAGLQIA-OH
编号: | 151113 |
中文名称: | Flagelin 22、Flg22, Flagellin Fragment |
英文名: | Flagelin 22、Flg22, Flagellin Fragment |
CAS号: | 304642-91-9 |
单字母: | H2N-QRLSTGSRINSAKDDAAGLQIA-OH |
三字母: | H2N N端氨基 -Gln谷氨酰胺 -Arg精氨酸 -Leu亮氨酸 -Ser丝氨酸 -Thr苏氨酸 -Gly甘氨酸 -Ser丝氨酸 -Arg精氨酸 -Ile异亮氨酸 -Asn天冬酰胺 -Ser丝氨酸 -Ala丙氨酸 -Lys赖氨酸 -Asp天冬氨酸 -Asp天冬氨酸 -Ala丙氨酸 -Ala丙氨酸 -Gly甘氨酸 -Leu亮氨酸 -Gln谷氨酰胺 -Ile异亮氨酸 -Ala丙氨酸 -OHC端羧基 |
氨基酸个数: | 22 |
分子式: | C93H162N32O34 |
平均分子量: | 2272.48 |
精确分子量: | 2271.19 |
等电点(PI): | 11.48 |
pH=7.0时的净电荷数: | 1.98 |
平均亲水性: | 0.345 |
疏水性值: | -0.48 |
消光系数: | - |
来源: | 人工化学合成,仅限科学研究使用,不得用于人体。 |
储存条件: | 负80℃至负20℃ |
标签: | 多肽标签(Tag) 细菌肽(Bacterial Peptides) |
flg22 Peptide能够诱导植物的天然免疫性。
Flagelin 22 (Flagellin 22) 是细菌鞭毛素的一个片段,在植物和藻类中都是有效的诱导剂。
Flagelin 22 (Flagellin 22), a fragment of bacterial flagellin, is an effective elicitor in both plants and algae.
专肽生物可以提供常见抗体制备、免疫学研究等生物科研需求的各类标签多肽(Tag peptide)以及蛋白底物产品,可用荧光标记(FITC、5-Fam)等。除常见抗体中抗原决定部位的标签多肽(tag: His | c-Myc | HA | FLAG | V5 | X-press | VSV tag | T7 tag | Snoop tag | E-tag | Sof tag 1 | Spy tag |等等),我们还提供各类定制标签肽的合成,并可提供荧光标记(FITC、5-Fam)等。同时,免费提供的MS和HPLC分析报告。
Definition
Bacterial peptides are protein fragments which are either part of a bacterium or produced by a bacteria1.
Classification
Different classes of peptides are produced by bacteria. Some examples include, antibiotics, enterotoxins, flagellar proteins, lipoproteins and various enzymes1.
Structural Characteristics
Structural characteristics of some bacterial peptides are described below-
A) Malaria merozoite surface peptide (MSP-1): It is synthesized as a large precursor on the surface of the bacterium Plasmodium falciparum. Proteolytic cleavage results in the production of a 19 KDa product whose tertiary structure is maintained by disulphide bridges2.
B) Giardia variable surface protein: This peptide is the specific conserved region of the Giardia variable surface proteins (VSPs) that are cysteine rich zinc finger proteins. VSPs differ in size and sequence, they are characterized by this highly conserved C-terminal membrane spanning region, a hydrophilic cytoplasmic tail with a conserved five amino acid CRGKA signature sequence3,4.
C) P.falciparum liver stage antigen 3: The protein is 200Kda and is highly conserved among parasites from different geographic regions5.
Mode of action
A) MSP-1 is known to trigger antibody response by CD4 helper T cells. It is likely that these cells bind to the C-terminal domain of MSP-12.
B) VSPs have a conserved hydrophilic amono acid trail that is palmitoyted by palmityl tranferases upon which they are activated3,4.
C) P. falciparum liver stage antigen 3 is a potent antigen that is recongnized by T cells5.
Functions
A) MSP-1 is a vaccine candidate for Plasmodium falciparum infection. It triggers a CD-4 T cell response2.
B) VSPs are necessary for survival in the environment and host infection3,4.
C) P.falciparum stage antigen 3 is also a good candidate vaccine as it activates both T and B cell responses5.
References
1. Gitai Z (2005). "The new bacterial cell biology: moving parts and subcellular architecture". Cell, 120 (5): 577–86.
2. Stuart JQ and Jean L (2001). Different regions of the malaria merozoite surface protein 1 of Plasmodium chabaudi elicit distinct T-cell and antibody isotype responses. Infect Immun, 69(4): 2245–2251.
3. Davids BJ, Reiner DS, Birkeland SR, Preheim SP, Cipriano MJ, McArthur AG, Gillin FD (2006). A New Family of Giardial Cysteine-Rich Non-VSP Protein Genes and a Novel Cyst Protein. PLoS ONE, 20,1:e44.
4. Touz MC, Conrad JT, Nash TE (2005). A novel palmitoyl acyl transferase controls surface protein palmitoylation and cytotoxicity in Giardia lamblia. Mol Microbiol., 58 (4), 999-1011.
5. Jean-Pierre S, Blanca LP, Karima B, Pierra D, Pierra D (2001). DNA Immunization by Plasmodium falciparum liver-stage antigen 3 induces protection against Plasmodium yoelii Sporozoite challenge. Infect Immun., 69, 1202–1206.
Bojun Lu, et al. Defense responses in female gametophytes of Saccharina japonica (Phaeophyta) induced by flg22-derived peptides. Journal of Applied Phycology (2016), 28(3), 1793-1801. : https://link.springer.com/article/10.1007/s10811-015-0721-3
Bojun Lu, et al. Defense responses in female gametophytes of Saccharina japonica (Phaeophyta) induced by flg22-derived peptides. Journal of Applied Phycology (2016), 28(3), 1793-1801. : https://link.springer.com/article/10.1007/s10811-015-0721-3