400-998-5282
专注多肽 服务科研
400-998-5282
专注多肽 服务科研
一种HCV蛋白酶底物，在残基P1和P1之间结合酯键。由于剪切键与酰基酶中间体的酯交换反应很快，这种底物显示出非常高的kcat/Km值，能够检测亚纳米非结构蛋白3（NS3蛋白酶）浓度的活性。
编号：587540
CAS号：
单字母：Ac-DE-D(Edans)-EE-Abu-OAla-S-K(Dabcyl)-CONH2
| 编号: | 587540 |
| 中文名称: | HCV蛋白酶底物：HCV Protease FRET Substrate (RET S1) |
| 英文名: | HCV Protease FRET Substrate (RET S1) |
| 英文同义词: | Ac-Asp-Glu-Asp(EDANS)-Glu-Glu-Abu-psi-(COO)Ala-Ser-Lys(DABCYL)-NH2 |
| 单字母: | Ac-DE-D(Edans)-EE-Abu-OAla-S-K(Dabcyl)-CONH2 |
| 三字母: | Ac N端乙酰化封端，一种常见的修饰方式，常用于模拟蛋白质中的肽片段。 -AspL-天冬氨酸：aspartic acid。系统命名为(2S)-氨基-丁二酸。是编码氨基酸，又是神经递质。符号：D，Asp。D-天冬氨酸存在于多种细菌的细胞壁和短杆菌肽A中。 -GluL-谷氨酸：glutamic acid。系统命名为(2S)-氨基-戊二酸。是编码氨基酸。符号：E，Glu。D-谷氨酸存在于多种细菌的细胞壁和某些细菌杆菌肽中。 -Asp(Edans)天冬氨酸的侧链标记Edans基团 -GluL-谷氨酸：glutamic acid。系统命名为(2S)-氨基-戊二酸。是编码氨基酸。符号：E，Glu。D-谷氨酸存在于多种细菌的细胞壁和某些细菌杆菌肽中。 -GluL-谷氨酸：glutamic acid。系统命名为(2S)-氨基-戊二酸。是编码氨基酸。符号：E，Glu。D-谷氨酸存在于多种细菌的细胞壁和某些细菌杆菌肽中。 -AbuL-α-氨基丁酸（Abu）是一种非蛋白源性的α氨基酸。Abu是丙氨酸的同源物，含有一个额外的亚甲基（-CH2-）基团。 -OAlaAla左侧形成酯键 -SerL-丝氨酸：serine。系统命名为(2S)-氨基-3-羟基丙酸。是编码氨基酸。因可从蚕丝中获得而得名。符号：S，Ser。在丝原蛋白及某些抗菌素中含有 D-丝氨酸。 -Lys(Dabcyl)赖氨酸侧链标记Dabcyl -CONH2C端酰胺化 |
| 氨基酸个数: | 9 |
| 分子式: | C58H89O25N15S1 |
| 平均分子量: | 1428.48 |
| 精确分子量: | 1427.59 |
| 等电点(PI): | - |
| pH=7.0时的净电荷数: | -4 |
| 平均亲水性: | 2.46 |
| 疏水性值: | -2.96 |
| 消光系数: | - |
| 标签: | 氨基酸衍生物肽 DABCYL标记肽 EDANS修饰肽 |
| 参考文献（References）: | Lin, C. et al. J. Biol. Chem. 10, 1074 (2004) Kakiuchi, N. et al. J. Virol. Methods 80, 77 (1999) Liu, Y. et al. Anal. Biochem. 267, 331 (1999) Taliani, M. et al. Anal. Biochem. 240, 60 (1996) |
一种HCV蛋白酶底物，在残基P1和P1之间结合酯键。由于剪切键与酰基酶中间体的酯交换反应很快，这种底物显示出非常高的kcat/Km值，能够检测亚纳米非结构蛋白3（NS3蛋白酶）浓度的活性。它被广泛用于NS3蛋白酶活性的连续测定。底物切割与酶浓度成正比，NS3的检测限在1 nM至250 pM之间。切割该底物后，可以在Abs/Em=355/500 nM处监测荧光。作为一种内部测序的荧光底物，它可用于监测HCV NS3蛋白酶活性。
A HCV protease substrate that incoporates an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm. As an internally quenced fluorogenic substrate, it can be used to monitor HCV NS3 protease activity.
DABCYL标记说明
4-(4-二甲氨基苯基偶氮)苯甲酰基（Dabcyl）是一种发色团，常与荧光团（如EDANS）结合用作猝灭剂。Dabcyl/EDANS FRET（荧光共振能量转移）对的福斯特半径为3.3纳米。
4-(4-Dimethylaminophenylazo)benzoyl (Dabcyl) is a chromophor often used as a quencher in conjunction with a fluorophore (e.g., EDANS). Dabcyl/EDANS FRET pair have a Forster radius of 3.3 nm.
DABCYL Related Articles:
Single-cell assays using integrated continuous-flow microfluidics.
Ng, Ee Xien, Myat Noe Hsu, Guoyun Sun, and Chia-Hung Chen. Methods in Enzymology 628 (2019): 59-94.
Characterization of the Altai Maral Chymosin Gene, Production of a Chymosin Recombinant Analog in the Prokaryotic Expression System, and Analysis of Its Several Biochemical Properties.
Belenkaya, S. V., A. A. Bondar, T. A. Kurgina, V. V. Elchaninov, A. Yu Bakulina, E. A. Rukhlova, O. I. Lavrik, A. A. Ilyichev, and D. N. Shcherbakov. Biochemistry (Moscow), 2020.
Urinary detection of early responses to checkpoint blockade and of resistance to it via protease-cleaved antibody-conjugated sensors.
Mac, Quoc D., Anirudh Sivakumar, Hathaichanok Phuengkham, Congmin Xu, James R. Bowen, Fang-Yi Su, Samuel Z. Stentz et al. Nature Biomedical Engineering (2022): 1-15.
Glossary: EDANS
5-[(2-Aminoethyl)amino]naphthalene-1-sulfonyl (EDANS) is fluorophor with an excitation at 340 nm ▉ and emission of 490 nm ▉. EDANS is often paired together with acceptors like dabcyl for FRET experiements.
Properties of Hemoglobin Decolorized with a Histidine-Specific Protease.
Shi, Jing, et al. Journal of Food Science 80.6 (2015): E1202-E1208.
EDANS标记说明
5-[(2-Aminoethyl)amino]naphthalene-1-sulfonyl (EDANS) is fluorophore with an excitation at 340 nm ▉ and emission of 490 nm ▉. EDANS are often paired together with acceptors like dabcyl for FRET experiments.
