400-998-5282
专注多肽 服务科研
400-998-5282
专注多肽 服务科研
汗腺中表达的蛋白质dermcidin被分泌到汗液中并运输到表皮表面。蛋白质的伴随加工产生广谱抗菌 47 个氨基酸肽，dermcidin-1 (DCD-1)。Dermcidin 和天然存在的片段如 DCD-1L 不会扰乱膜渗透性并与不同的表面分子结合
编号：200088
CAS号：478898-18-9
单字母：H2N-SSLLEKGLDGAKKAVGGLGKLGKDAVEDLESVGKGAVHDVKDVLDSVL-OH
Dermcidin-1L (human) 是一种由汗腺分泌的抗生素肽。Dermcidin-1L (human) 具有抗菌活性。Dermcidin-1L (human) 可用于炎症性皮肤病的研究。
抗菌肽是众多物种先天免疫反应的重要组成部分。Dermcidin: a novel human antibiotic peptide secreted by sweat glands 报道了Dermcidin基因的分离研究，该基因编码的抗菌肽具有广谱抗菌活性，且与其他已知抗菌肽无同源性。这种蛋白质在汗腺中特异性持续表达，分泌至汗液并转运至表皮表面。在汗液环境中，经蛋白酶处理后生成的47个氨基酸肽段对多种病原微生物表现出抗菌活性。该肽段在pH值范围较宽、高盐浓度条件下仍能保持活性，这与人体汗液环境条件高度吻合。这表明汗液通过抗菌肽的存在参与调控人体皮肤菌群。该肽段可能在细菌定植后的最初几小时内，通过抑制潜在病原体感染发挥保护作用。
在汗腺中表达的去皮蛋白被分泌到汗液中并被运输到表皮表面。该蛋白的伴随加工产生广谱抗微生物47个氨基酸肽，dermcidin-1 (DCD-1)。去皮素和自然产生的片段如DCD-1L不会扰乱膜的通透性，并与不同的表面分子结合。Niyonsaba等人研究报道，DCD-1L刺激角质形成细胞产生细胞因子和趋化因子，如TNF-α、IL-8、CXCL10和CCL20
The protein dermcidin expressed in the sweat glands is secreted into the sweat and transported to the epidermal surface. The concomitant processing of the protein yields a broad-spectrum antimicrobial 47 amino acid peptide, dermcidin-1 (DCD-1). Dermcidin and naturally occurring fragments as DCD-1L do not perturb membrane permeability and bind to distinct surface molecules. Niyonsaba et al. have reported that DCD-1L stimulates keratinocytes to generate cytokines and chemokines such as TNF-α, IL-8, CXCL10, and CCL20.
参考文献
Schittek B, et al. Dermcidin: a novel human antibiotic peptide secreted by sweat glands. Nat Immunol. 2001 Dec;2(12):1133-7
| DOI | 名称 | |
|---|---|---|
| 10.1128/AAC.00181-06 | Naturally processed dermcidin-derived peptides do not permeabilize bacterial membranes and kill microorganisms irrespective of their charge | 下载 |
| 10.1111/j.1365-2133.2008.08925.x | The human antimicrobial peptide dermcidin activates normal human keratinocytes | 下载 |
多肽H2N-Ser-Ser-Leu-Leu-Glu-Lys-Gly-Leu-Asp-Gly-Ala-Lys-Lys-Ala-Val-Gly-Gly-Leu-Gly-Lys-Leu-Gly-Lys-Asp-Ala-Val-Glu-Asp-Leu-Glu-Ser-Val-Gly-Lys-Gly-Ala-Val-His-Asp-Val-Lys-Asp-Val-Leu-Asp-Ser-Val-Leu-COOH的合成步骤：
1、合成CTC树脂：称取2.87g CTC Resin（如初始取代度约为0.68mmol/g）和2.34mmol Fmoc-Leu-OH于反应器中，加入适量DCM溶解氨基酸（需要注意，此时CTC树脂体积会增大好几倍，避免DCM溶液过少），再加入5.85mmol DIPEA（Mw：129.1,d：0.740g/ml），反应2-3小时后，可不抽滤溶液，直接加入1ml的HPLC级甲醇，封端半小时。依次用DMF洗涤2次，甲醇洗涤1次，DCM洗涤一次，甲醇洗涤一次，DCM洗涤一次，DMF洗涤2次（这里使用甲醇和DCM交替洗涤，是为了更好地去除其他溶质，有利于后续反应）。得到 Fmoc-Leu-CTC Resin。结构图如下：
2、脱Fmoc：加3倍树脂体积的20%Pip/DMF溶液，鼓氮气30分钟，然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Leu-CTC Resin 。（此步骤脱除Fmoc基团，茚三酮检测为蓝色，Pip为哌啶）。结构图如下：
3、缩合：取5.85mmol Fmoc-Val-OH 氨基酸，加入到上述树脂里，加适当DMF溶解氨基酸，再依次加入11.71mmol DIPEA，5.56mmol HBTU。反应30分钟后，取小样洗涤，茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。得到Fmoc-Val-Leu-CTC Resin。氨基酸：DIPEA：HBTU：树脂=3：6：2.85：1（摩尔比）。结构图如下：
4、依次循环步骤二、步骤三，依次得到
H2N-Val-Leu-CTC Resin
Fmoc-Ser(tBu)-Val-Leu-CTC Resin
H2N-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Ala-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Ala-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Gly-Ala-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Gly-Ala-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Asp(OtBu)-Gly-Ala-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Asp(OtBu)-Gly-Ala-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Leu-Asp(OtBu)-Gly-Ala-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Leu-Asp(OtBu)-Gly-Ala-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Gly-Leu-Asp(OtBu)-Gly-Ala-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Gly-Leu-Asp(OtBu)-Gly-Ala-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Lys(Boc)-Gly-Leu-Asp(OtBu)-Gly-Ala-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Lys(Boc)-Gly-Leu-Asp(OtBu)-Gly-Ala-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Glu(OtBu)-Lys(Boc)-Gly-Leu-Asp(OtBu)-Gly-Ala-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Glu(OtBu)-Lys(Boc)-Gly-Leu-Asp(OtBu)-Gly-Ala-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Leu-Glu(OtBu)-Lys(Boc)-Gly-Leu-Asp(OtBu)-Gly-Ala-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Leu-Glu(OtBu)-Lys(Boc)-Gly-Leu-Asp(OtBu)-Gly-Ala-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Leu-Leu-Glu(OtBu)-Lys(Boc)-Gly-Leu-Asp(OtBu)-Gly-Ala-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Leu-Leu-Glu(OtBu)-Lys(Boc)-Gly-Leu-Asp(OtBu)-Gly-Ala-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Ser(tBu)-Leu-Leu-Glu(OtBu)-Lys(Boc)-Gly-Leu-Asp(OtBu)-Gly-Ala-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
H2N-Ser(tBu)-Leu-Leu-Glu(OtBu)-Lys(Boc)-Gly-Leu-Asp(OtBu)-Gly-Ala-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
Fmoc-Ser(tBu)-Ser(tBu)-Leu-Leu-Glu(OtBu)-Lys(Boc)-Gly-Leu-Asp(OtBu)-Gly-Ala-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin
以上中间结构，均可在专肽生物多肽计算器-多肽结构计算器中，一键画出。
最后再经过步骤二得到 H2N-Ser(tBu)-Ser(tBu)-Leu-Leu-Glu(OtBu)-Lys(Boc)-Gly-Leu-Asp(OtBu)-Gly-Ala-Lys(Boc)-Lys(Boc)-Ala-Val-Gly-Gly-Leu-Gly-Lys(Boc)-Leu-Gly-Lys(Boc)-Asp(OtBu)-Ala-Val-Glu(OtBu)-Asp(OtBu)-Leu-Glu(OtBu)-Ser(tBu)-Val-Gly-Lys(Boc)-Gly-Ala-Val-His(Trt)-Asp(OtBu)-Val-Lys(Boc)-Asp(OtBu)-Val-Leu-Asp(OtBu)-Ser(tBu)-Val-Leu-CTC Resin，结构如下：
5、切割：6倍树脂体积的切割液（或每1g树脂加8ml左右的切割液），摇床摇晃 2小时，过滤掉树脂，用冰无水乙醚沉淀滤液，并用冰无水乙醚洗涤沉淀物3次，最后将沉淀物放真空干燥釜中，常温干燥24小试，得到粗品H2N-Ser-Ser-Leu-Leu-Glu-Lys-Gly-Leu-Asp-Gly-Ala-Lys-Lys-Ala-Val-Gly-Gly-Leu-Gly-Lys-Leu-Gly-Lys-Asp-Ala-Val-Glu-Asp-Leu-Glu-Ser-Val-Gly-Lys-Gly-Ala-Val-His-Asp-Val-Lys-Asp-Val-Leu-Asp-Ser-Val-Leu-COOH。结构图见产品结构图。
切割液选择：1）TFA：H2O=95%：5%
2）TFA：H2O：TIS=95%：2.5%：2.5%
3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%
（前两种适合没有容易氧化的氨基酸，例如Trp、Cys、Met。第三种适合几乎所有的序列。）
6、纯化冻干：使用液相色谱纯化，收集目标峰液体，进行冻干，获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度 是否目标纯度。
7、最后总结：
杭州专肽生物技术有限公司（ALLPEPTIDE https://www.allpeptide.com）主营定制多肽合成业务，提供各类长肽，短肽，环肽，提供各类修饰肽，如：荧光标记修饰（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基团修饰肽（叠氮、炔基、DBCO、DOTA、NOTA等），同位素标记肽（N15、C13），订书肽（Stapled Peptide）,脂肪酸修饰肽（Pal、Myr、Ste），磷酸化修饰肽（P-Ser、P-Thr、P-Tyr），环肽（酰胺键环肽、一对或者多对二硫键环），生物素标记肽，PEG修饰肽，甲基化修饰肽等。
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