400-998-5282
专注多肽 服务科研
400-998-5282
专注多肽 服务科研
编号：200149
CAS号：90599-39-6
单字母：H2N-YADAIFTNSYRKVLGQLSARKLLQDIMSRQQGESNQERGARARL-OH
GRF is the hypothalamic peptide hormone that specifically stimulates synthesis and release of the growth hormone by somatotropic cells of the anterior pituitary gland.
定义
生长激素（GH）或生长激素在垂体的前部产生。GH的许多作用是由肝脏和其他组织分泌的胰岛素样生长因子1（IGF-1）介导的。
发现
生长激素释放激素（GHRH）基因产生的前体分子包含GHRH和被称为GHRH相关肽（GHRH-RP）的羧基末端肽。像GHRH一样，这种肽在体外和体内刺激干细胞因子（SCF）的表达，干细胞因子是一种重要的生殖和造血细胞因子。Steinmetz等人在2000年使用大鼠Sertoli细胞的原代培养物比较了GHRH-RP和GHRH 1处理后的作用时间和SCF刺激水平。生长激素释放激素从神经分泌神经末梢释放，并由下丘脑-垂体门系统携带至垂体前叶腺。它通过刺激生长激素释放激素受体2刺激GH分泌。在两栖性下丘脑中发现了一种新的Arg-Phe-NH 2（RFamide）肽。含有该肽的细胞体和末端分别位于视交叉上核和中位隆起。这种肽被进一步显示出具有相当大的生长激素（GH） -释放活性在体外和体内，并因此命名为青蛙GH释放肽（FGRP）3。
结构特征
GH或生长激素是在垂体1的前部产生的191个氨基酸的蛋白质激素。人胎盘催乳素（hPL），生长激素和催乳激素（PRL）构成生长激素家族。它们都具有约200个氨基酸，2个二硫键，并且没有糖基化作用。尽管每种都具有特殊的受体并具有独特的活性特征，但它们都具有促进生长和生乳的活性。成熟的GH（22,000道尔顿）在嗜酸性垂体生长激素中合成为单个多肽链4，5。编码fGRP前体多肽的cDNA的分子克隆表明，它编码fGRP及其推定的基因相关肽（fGRP-RP-1，-RP-2和-RP-3）。随后，作者将这些推定的fGRP-RPs鉴定为成熟肽，并分析了它们的促生理活性。在体外和体内，只有fGRP-RP-2刺激GH和催乳激素（PRL）的释放。因此，除了fGRP，fGRP-RP-2还充当青蛙垂体的下丘脑因子，刺激GH和PRL 3的释放。
行动方式
GH或生长激素的释放受两种激素的相反作用控制：生长激素释放激素，它既刺激生长激素的合成又分泌；和生长抑素，抑制其释放。GH在人体几乎每个器官和系统的几个复杂生理过程的调节中都起着重要作用。它促进软组织，软骨和骨骼的生长；增加蛋白质，RNA和DNA的合成；减少碳水化合物的利用并增加脂肪的利用。GH通过对增殖性软骨细胞或干细胞软骨细胞的分化产生刺激作用来促进骨生长。GH的许多作用是由肝脏和其他组织分泌的胰岛素样生长因子1（IGF-1）介导的。生长激素的过量产生会导致青年人的巨人症和成年人的肢端肥大症。儿童缺乏激素会导致侏儒症4、5。在人类中，生长激素促进糖异生，因此是高血糖的。它促进细胞摄取氨基酸，结果是GH疗法使生物体处于正氮平衡，这与成长中的儿童相似。
功能
生长激素释放激素 还直接促进慢波睡眠。正常的产后生长，骨骼生长，对蛋白质，碳水化合物和脂质代谢的调节作用都需要生长激素1。
遗传缺陷， 与GH相关的遗传缺陷很多。缺乏GH的矮人缺乏合成或分泌GH的能力，这些矮个子的个体对GH治疗反应良好。侏儒缺乏对GH的IGF-1反应，但缺乏其代谢作用。因此，在侏儒症中，缺乏症本质上是后受体。
肢端肥大症， 这些个体的缺陷显然与无法通过产生IGF-1对GH产生反应有关。长骨的骨closure闭合前产生过量的GH导致巨人症，而骨epi闭合后GH过量时，骨的生长导致肢端肥大症的特征5。
脂肪分解，生长激素是脂肪分解，诱导组织脂类的分解，从而提供用于支持通过增加的氨基酸摄取引起的刺激蛋白质合成的能源供应。
参考
1. Steinmetz R, Lazzaro N, Rothrock JK, Pescovitz OH (2000). Effects of growth hormone-releasing hormone-related peptide on stem cell factor expression in cultured rat sertoli cells. Journal Endocrine, 12(3):323-327.
2. Obál F, Krueger J (2001). The somatotropic axis and sleep. Rev Neurol., 157(11):12-15.
3. Ukena K, Koda A, Yamamoto K, Iwakoshi-Ukena E, Minakata H, Kikuyama S, Tsutsui K (2006). Structures and diverse functions of frog growth hormone-releasing peptide (fGRP) and its related peptides (fGRP-RPs): a review. Journal of Experimental Zoology Part A: Comparative Experimental Biology, 305(9):815-821.
4. Englander EW, Gomez GA, Greeley GH Jr (2004). Alterations in stomach ghrelin production and in ghrelin-induced growth hormone secretion in the aged rat. Mech. Ageing Dev., 125(12):871-875.
5. Sonntag WE, Lynch C, Thornton P, Khan A, Bennett S, Ingram R (2000). The effects of growth hormone and IGF-1 deficiency on cerebrovascular and brain ageing. J. Anat., 197(4):575-585.
| DOI | 名称 | |
|---|---|---|
| 10.1507/endocrj1954.32.907 | Production of antisera to growth hormone-releasing factor: usefulness in radioimmunoassay and passive immunization | 下载 |
多肽H2N-Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-Gln-Gln-Gly-Glu-Ser-Asn-Gln-Glu-Arg-Gly-Ala-Arg-Ala-Arg-Leu-COOH的合成步骤：
1、合成CTC树脂：称取2.91g CTC Resin（如初始取代度约为0.98mmol/g）和3.42mmol Fmoc-Leu-OH于反应器中，加入适量DCM溶解氨基酸（需要注意，此时CTC树脂体积会增大好几倍，避免DCM溶液过少），再加入8.56mmol DIPEA（Mw：129.1,d：0.740g/ml），反应2-3小时后，可不抽滤溶液，直接加入1ml的HPLC级甲醇，封端半小时。依次用DMF洗涤2次，甲醇洗涤1次，DCM洗涤一次，甲醇洗涤一次，DCM洗涤一次，DMF洗涤2次（这里使用甲醇和DCM交替洗涤，是为了更好地去除其他溶质，有利于后续反应）。得到 Fmoc-Leu-CTC Resin。结构图如下：
2、脱Fmoc：加3倍树脂体积的20%Pip/DMF溶液，鼓氮气30分钟，然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Leu-CTC Resin 。（此步骤脱除Fmoc基团，茚三酮检测为蓝色，Pip为哌啶）。结构图如下：
3、缩合：取8.56mmol Fmoc-Arg(Pbf)-OH 氨基酸，加入到上述树脂里，加适当DMF溶解氨基酸，再依次加入17.11mmol DIPEA，8.13mmol HBTU。反应30分钟后，取小样洗涤，茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。得到Fmoc-Arg(Pbf)-Leu-CTC Resin。氨基酸：DIPEA：HBTU：树脂=3：6：2.85：1（摩尔比）。结构图如下：
4、依次循环步骤二、步骤三，依次得到
H2N-Arg(Pbf)-Leu-CTC Resin
Fmoc-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Arg(Pbf)-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Arg(Pbf)-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Asn(Trt)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Asn(Trt)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Thr(tBu)-Asn(Trt)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Thr(tBu)-Asn(Trt)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Phe-Thr(tBu)-Asn(Trt)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Phe-Thr(tBu)-Asn(Trt)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Ile-Phe-Thr(tBu)-Asn(Trt)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Ile-Phe-Thr(tBu)-Asn(Trt)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Ala-Ile-Phe-Thr(tBu)-Asn(Trt)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Ala-Ile-Phe-Thr(tBu)-Asn(Trt)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Asp(OtBu)-Ala-Ile-Phe-Thr(tBu)-Asn(Trt)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Asp(OtBu)-Ala-Ile-Phe-Thr(tBu)-Asn(Trt)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Ala-Asp(OtBu)-Ala-Ile-Phe-Thr(tBu)-Asn(Trt)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
H2N-Ala-Asp(OtBu)-Ala-Ile-Phe-Thr(tBu)-Asn(Trt)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
Fmoc-Tyr(tBu)-Ala-Asp(OtBu)-Ala-Ile-Phe-Thr(tBu)-Asn(Trt)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin
以上中间结构，均可在专肽生物多肽计算器-多肽结构计算器中，一键画出。
最后再经过步骤二得到 H2N-Tyr(tBu)-Ala-Asp(OtBu)-Ala-Ile-Phe-Thr(tBu)-Asn(Trt)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Val-Leu-Gly-Gln(Trt)-Leu-Ser(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-Gln(Trt)-Asp(OtBu)-Ile-Met-Ser(tBu)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Ser(tBu)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Arg(Pbf)-Gly-Ala-Arg(Pbf)-Ala-Arg(Pbf)-Leu-CTC Resin，结构如下：
5、切割：6倍树脂体积的切割液（或每1g树脂加8ml左右的切割液），摇床摇晃 2小时，过滤掉树脂，用冰无水乙醚沉淀滤液，并用冰无水乙醚洗涤沉淀物3次，最后将沉淀物放真空干燥釜中，常温干燥24小试，得到粗品H2N-Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-Gln-Gln-Gly-Glu-Ser-Asn-Gln-Glu-Arg-Gly-Ala-Arg-Ala-Arg-Leu-COOH。结构图见产品结构图。
切割液选择：1）TFA：H2O=95%：5%
2）TFA：H2O：TIS=95%：2.5%：2.5%
3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%
（前两种适合没有容易氧化的氨基酸，例如Trp、Cys、Met。第三种适合几乎所有的序列。）
6、纯化冻干：使用液相色谱纯化，收集目标峰液体，进行冻干，获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度 是否目标纯度。
7、最后总结：
杭州专肽生物技术有限公司（ALLPEPTIDE https://www.allpeptide.com）主营定制多肽合成业务，提供各类长肽，短肽，环肽，提供各类修饰肽，如：荧光标记修饰（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基团修饰肽（叠氮、炔基、DBCO、DOTA、NOTA等），同位素标记肽（N15、C13），订书肽（Stapled Peptide）,脂肪酸修饰肽（Pal、Myr、Ste），磷酸化修饰肽（P-Ser、P-Thr、P-Tyr），环肽（酰胺键环肽、一对或者多对二硫键环），生物素标记肽，PEG修饰肽，甲基化修饰肽等。
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