该乙酰化肽是膜联蛋白1（ANXA1）的氨基酸2至28的N-末端片段。
编号：434203
CAS号：
单字母：Ac-AMVSEFLKQAWIENEEQEYVVQTVK-OH
| 编号: | 434203 |
| 中文名称: | 膜联蛋白Annexin 1 (ANXA - 1, Ac 2 - 26) |
| 英文名: | Annexin 1 (ANXA - 1, Ac 2 - 26) |
| 单字母: | Ac-AMVSEFLKQAWIENEEQEYVVQTVK-OH |
| 三字母: | Ac N端乙酰化封端 -Ala丙氨酸 -Met甲硫氨酸 -Val缬氨酸 -Ser丝氨酸 -Glu谷氨酸 -Phe苯丙氨酸 -Leu亮氨酸 -Lys赖氨酸 -Gln谷氨酰胺 -Ala丙氨酸 -Trp色氨酸 -Ile异亮氨酸 -Glu谷氨酸 -Asn天冬酰胺 -Glu谷氨酸 -Glu谷氨酸 -Gln谷氨酰胺 -Glu谷氨酸 -Tyr酪氨酸 -Val缬氨酸 -Val缬氨酸 -Gln谷氨酰胺 -Thr苏氨酸 -Val缬氨酸 -Lys赖氨酸 -OHC端羧基 |
| 氨基酸个数: | 25 |
| 分子式: | C137H210O44N32S1 |
| 平均分子量: | 3041.39 |
| 精确分子量: | 3039.49 |
| 等电点(PI): | 4.28 |
| pH=7.0时的净电荷数: | -1.99 |
| 平均亲水性: | 0.02 |
| 疏水性值: | -0.37 |
| 消光系数: | 6990 |
| 标签: | 膜联蛋白(Annexin) |
该乙酰化肽是膜联蛋白1（ANXA1）的氨基酸2至28的N-末端片段。ANXA1，以前称为脂皮质素1或LC1，是膜联蛋白超家族的一员，具有强大的白细胞抗迁移活性。该片段以及全长ANXA1可以显著减少小鼠中多形核白细胞（PMN）的流入。ANXA1和ANXA1模拟物，如Ac（2-12），在急性和慢性炎症中抑制中性粒细胞的募集。这种肽能够抑制中性粒细胞外渗。
This acetylated peptide is amino acids 2 to 28 N-terminus fragment of Annexin 1 (ANXA1). ANXA1, previously referred to as lipocortin 1 or LC1, is a member of the annexin superfamily that has potent leukocyte antimigratory activity. This fragment, as well as the full-length ANXA1, may significantly reduce polymorphonuclear leukocyte (PMN) influx in mice. ANXA1 and ANXA1 mimetics, such as Ac (2-12), inhibit recruitment of neutrophils in acute and chronic inflammation. This peptide is able to inhibit neutrophil extravasation.
Definition
Annexins are calcium-dependent phospholipid-binding proteins. More than a thousand proteins of the annexin superfamily have been identified in major eukaryotic phyla, but annexins are absent from yeasts and prokaryotes.
Discovery
The first annexin to be identified was annexin VII (synexin) from the bovine adrenal medulla by creutz and team in 19781.
Classification
Annexins are grouped under 5 major classes (A-E), the 12 annexins which are commonly found in vertebrates are classified under annexin A family and named as annexins A1-A13 (or ANXA1-ANXA13), leaving A12 not assigned in the official nomenclature. Annexins outside vertebrates are classified under family B (in invertebrates), C (in fungi and under groups of unicellular eukaryotes), D (in plants), and E (in protists).
Structural Characteristics
Each annexin is composed of two principal domains: the divergent NH2-terminal “head” and the conserved COOH-terminal protein core. The COOH-terminal protein core harbors the Ca2+ and membrane binding sites and is responsible for mediating the canonical membrane binding properties. An annexin core comprises four (in annexin A6 eight) segments of internal and inter annexin homology that are easily identified in a linear sequence alignment. It forms a highly alpha helical and tightly packed disk with a slight curvature and two principle sides. The more convex side contains Ca2+ binding sites, the so-called type II and type III sites, and faces the membrane when an annexin is associated peripherally with phospholipids2. Many annexins undergo posttranslational modifications viz., phosphorylation and myristoylation1.
Mode of action
The process of membrane aggregation requires the self association of annexin molecules where Ca2+ plays an important role. In case of synexin (annexin VII) at lower concentration of Ca2+ level it is soluble, if the concentration of Ca2+ increase it results in binding of annexins to membrane further increase in Ca2+ levels results in membrane aggregation and fusion.
Function
Annexins interact with various cell-membrane components that are involved in the structural organization of the cell, intracellular signaling by enzyme modulation and ion fluxes, growth control, and they can act as atypical calcium channels1.
References
1. Moss SE, Morgan RO (2004). The Annexins. Genome Biol., 5(4): 219.
