400-998-5282
专注多肽 服务科研
400-998-5282
专注多肽 服务科研
GRF是下丘脑肽激素，可特异性刺激垂体前叶的生长激素细胞合成和释放生长激素。
编号：143987
CAS号：86472-71-1
单字母：H2N-HADAIFTSSYRRILGQLYARKLLHEIMNRQQGERNQEQRSRFN-OH
| 参考文献（References）: | J. Spiess et al., Nature, 303, 532 (1983) |
GRF是下丘脑肽激素，可特异性刺激垂体前叶的生长激素细胞合成和释放生长激素。
GRF is the hypothalamic peptide hormone that specifically stimulates synthesis and release of the growth hormone by somatotropic cells of the anterior pituitary gland.
| DOI | 名称 | |
|---|---|---|
| 10.5483/bmbrep.2007.40.6.979 | Induction of growth hormone release by glycyrrhizae radix on rat | 下载 |
| 10.5483/bmbrep.2007.40.6.1016 | Induction of growth hormone release by dioscin from Dioscorea batatas DECNE | 下载 |
| 10.1371/journal.pone.0108456 | VGF changes during the estrous cycle: a novel endocrine role for TLQP peptides? | 下载 |
| 10.1371/journal.pone.0107388 | Butyrate increases intracellular calcium levels and enhances growth hormone release from rat anterior pituitary cells via the G-protein-coupled receptors GPR41 and 43 | 下载 |
| 10.1038/303532a0 | Characterization of rat hypothalamic growth hormone-releasing factor | 下载 |
| 10.1111/j.1399-3011.1993.tb00127.x | Development of a radioimmunoassay for some agonists of growth hormone-releasing hormone | 下载 |
多肽H2N-His-Ala-Asp-Ala-Ile-Phe-Thr-Ser-Ser-Tyr-Arg-Arg-Ile-Leu-Gly-Gln-Leu-Tyr-Ala-Arg-Lys-Leu-Leu-His-Glu-Ile-Met-Asn-Arg-Gln-Gln-Gly-Glu-Arg-Asn-Gln-Glu-Gln-Arg-Ser-Arg-Phe-Asn-COOH的合成步骤：
1、合成CTC树脂：称取1.95g CTC Resin（如初始取代度约为0.35mmol/g）和0.82mmol Fmoc-Asn(Trt)-OH于反应器中，加入适量DCM溶解氨基酸（需要注意，此时CTC树脂体积会增大好几倍，避免DCM溶液过少），再加入2.05mmol DIPEA（Mw：129.1,d：0.740g/ml），反应2-3小时后，可不抽滤溶液，直接加入1ml的HPLC级甲醇，封端半小时。依次用DMF洗涤2次，甲醇洗涤1次，DCM洗涤一次，甲醇洗涤一次，DCM洗涤一次，DMF洗涤2次（这里使用甲醇和DCM交替洗涤，是为了更好地去除其他溶质，有利于后续反应）。得到 Fmoc-Asn(Trt)-CTC Resin。结构图如下：
2、脱Fmoc：加3倍树脂体积的20%Pip/DMF溶液，鼓氮气30分钟，然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Asn(Trt)-CTC Resin 。（此步骤脱除Fmoc基团，茚三酮检测为蓝色，Pip为哌啶）。结构图如下：
3、缩合：取2.05mmol Fmoc-Phe-OH 氨基酸，加入到上述树脂里，加适当DMF溶解氨基酸，再依次加入4.09mmol DIPEA，1.95mmol HBTU。反应30分钟后，取小样洗涤，茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。得到Fmoc-Phe-Asn(Trt)-CTC Resin。氨基酸：DIPEA：HBTU：树脂=3：6：2.85：1（摩尔比）。结构图如下：
4、依次循环步骤二、步骤三，依次得到
H2N-Phe-Asn(Trt)-CTC Resin
Fmoc-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Arg(Pbf)-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Arg(Pbf)-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Tyr(tBu)-Arg(Pbf)-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Tyr(tBu)-Arg(Pbf)-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Ser(tBu)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Ser(tBu)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Thr(tBu)-Ser(tBu)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Thr(tBu)-Ser(tBu)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Ile-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Ile-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Ala-Ile-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Ala-Ile-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Asp(OtBu)-Ala-Ile-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Asp(OtBu)-Ala-Ile-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-Ala-Asp(OtBu)-Ala-Ile-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
H2N-Ala-Asp(OtBu)-Ala-Ile-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
Fmoc-His(Trt)-Ala-Asp(OtBu)-Ala-Ile-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin
以上中间结构，均可在专肽生物多肽计算器-多肽结构计算器中，一键画出。
最后再经过步骤二得到 H2N-His(Trt)-Ala-Asp(OtBu)-Ala-Ile-Phe-Thr(tBu)-Ser(tBu)-Ser(tBu)-Tyr(tBu)-Arg(Pbf)-Arg(Pbf)-Ile-Leu-Gly-Gln(Trt)-Leu-Tyr(tBu)-Ala-Arg(Pbf)-Lys(Boc)-Leu-Leu-His(Trt)-Glu(OtBu)-Ile-Met-Asn(Trt)-Arg(Pbf)-Gln(Trt)-Gln(Trt)-Gly-Glu(OtBu)-Arg(Pbf)-Asn(Trt)-Gln(Trt)-Glu(OtBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Arg(Pbf)-Phe-Asn(Trt)-CTC Resin，结构如下：
5、切割：6倍树脂体积的切割液（或每1g树脂加8ml左右的切割液），摇床摇晃 2小时，过滤掉树脂，用冰无水乙醚沉淀滤液，并用冰无水乙醚洗涤沉淀物3次，最后将沉淀物放真空干燥釜中，常温干燥24小试，得到粗品H2N-His-Ala-Asp-Ala-Ile-Phe-Thr-Ser-Ser-Tyr-Arg-Arg-Ile-Leu-Gly-Gln-Leu-Tyr-Ala-Arg-Lys-Leu-Leu-His-Glu-Ile-Met-Asn-Arg-Gln-Gln-Gly-Glu-Arg-Asn-Gln-Glu-Gln-Arg-Ser-Arg-Phe-Asn-COOH。结构图见产品结构图。
切割液选择：1）TFA：H2O=95%：5%
2）TFA：H2O：TIS=95%：2.5%：2.5%
3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%
（前两种适合没有容易氧化的氨基酸，例如Trp、Cys、Met。第三种适合几乎所有的序列。）
6、纯化冻干：使用液相色谱纯化，收集目标峰液体，进行冻干，获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度 是否目标纯度。
7、最后总结：
杭州专肽生物技术有限公司（ALLPEPTIDE https://www.allpeptide.com）主营定制多肽合成业务，提供各类长肽，短肽，环肽，提供各类修饰肽，如：荧光标记修饰（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基团修饰肽（叠氮、炔基、DBCO、DOTA、NOTA等），同位素标记肽（N15、C13），订书肽（Stapled Peptide）,脂肪酸修饰肽（Pal、Myr、Ste），磷酸化修饰肽（P-Ser、P-Thr、P-Tyr），环肽（酰胺键环肽、一对或者多对二硫键环），生物素标记肽，PEG修饰肽，甲基化修饰肽等。
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