专肽生物_定制多肽合成服务_多肽合成定制服务_多肽合成厂家_多肽合成公司

该肽对应于用于诱导大鼠实验性自身免疫性脑脊髓炎（EAE）的髓鞘碱性蛋白（MBP）脑源性表位。它对应于豚鼠MBP的氨基酸69-85。

This peptide corresponds to the myelin basic protein (MBP) encephalitogenic epitope used to induce experimental autoimmune encephalomyelitis (EAE) in rats. It corresponds to amino acids 69-85 from guinea pig MBP.

Peptide H-YGSLPQKSQRSQDENPV-OH is a Research Peptide with significant interest within the field academic and medical research. Recent citations using H-YGSLPQKSQRSQDENPV-OH include the following: Valproic acid ameliorates inflammation in experimental autoimmune encephalomyelitis rats Z Zhang , ZY Zhang, Y Wu, HJ Schluesener - Neuroscience, 2012 - Elsevierhttps://www.sciencedirect.com/science/article/pii/S0306452212007208 Lesional accumulation of RhoA+ cells in brains of experimental autoimmune encephalomyelitis and multiple sclerosis Z Zhang , J Schittenhelm, R Meyermann - Neuropathology and , 2008 - Wiley Online Libraryhttps://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2990.2007.00892.x The effect of electroacupuncture on T cell responses in rats with experimental autoimmune encephalitis Y Liu , X Liu, SS Bai, L Mu, Q Kong, B Sun - Journal of , 2010 - Elsevierhttps://www.sciencedirect.com/science/article/pii/S0165572809004883 Reciprocal effect of mesenchymal stem cell on experimental autoimmune encephalomyelitis is mediated by transforming growth factor-beta and interleukin-6 XJ Liu, JF Zhang, B Sun, HS Peng - Clinical & , 2009 - academic.oup.comhttps://academic.oup.com/cei/article-abstract/158/1/37/6438845 Epitope-specific immune tolerization ameliorates experimental autoimmune encephalomyelitis R Billetta, N Ghahramani, O Morrow, B Prakken - Clinical , 2012 - Elsevierhttps://www.sciencedirect.com/science/article/pii/S1521661612002069 Expansion of neonatal tolerance to self in adult life: I. The role of a bacterial adjuvant in tolerance spread N Grabie, I Wohl, S Youssef , G Wildbaum - International , 1999 - academic.oup.comhttps://academic.oup.com/intimm/article-abstract/11/6/899/707770 Cyclooxygenases-1 and-2 are differentially localized to microglia and endothelium in rat EAE and glioma MH Deininger, HJ Schluesener - Journal of neuroimmunology, 1999 - Elsevierhttps://www.sciencedirect.com/science/article/pii/S0165572898002574 Combined nasal administration of encephalitogenic myelin basic protein peptide 68-86 and IL-10 suppressed incipient experimental allergic encephalomyelitis in LY Xu, JS Yang, YM Huang, M Levi, H Link, BG Xiao - Clinical Immunology, 2000 - Elsevierhttps://www.sciencedirect.com/science/article/pii/S1521661600948952 Involvement of JAK/STAT signaling in the effect of cornel iridoid glycoside on experimental autoimmune encephalomyelitis amelioration in rats L Yin, Y Chen, Z Qu, L Zhang , Q Wang - Journal of , 2014 - Elsevierhttps://www.sciencedirect.com/science/article/pii/S0165572814001933 Interleukin-27 suppresses experimental autoimmune encephalomyelitis during bone marrow stromal cell treatment J Wang, G Wang, B Sun, H Li, L Mu, Q Wang, G Li - Journal of , 2008 - Elsevierhttps://www.sciencedirect.com/science/article/pii/S0896841107001114 Effects of autoantigen and dexamethasone treatment on expression of endothelial-monocyte activating polypeptide II and allograft-inflammatory factor-1 by activated HJ Schluesener, K Seid, R Meyermann - Acta neuropathologica, 1999 - Springerhttps://link.springer.com/article/10.1007/s004010050964 Therapeutic effects of nonerythropoietic erythropoietin analog ARA290 in experimental autoimmune encephalomyelitis rat H Chen, B Luo, X Yang, J Xiong, Z Liu, M Jiang - Journal of , 2014 - Elsevierhttps://www.sciencedirect.com/science/article/pii/S0165572814000095 IL-17 eliminates the therapeutic effects of myelin basic protein-induced nasal tolerance in experimental autoimmune encephalomyelitis by activating IL-6 GY Wang , B Sun, QF Kong, Y Zhang - Scandinavian , 2008 - Wiley Online Libraryhttps://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-3083.2008.02174.x Neutralizing antibodies to IFN-γ-inducing factor prevent experimental autoimmune encephalomyelitis G Wildbaum, S Youssef , N Grabie - The Journal of , 1998 - journals.aai.orghttps://journals.aai.org/jimmunol/article/161/11/6368/44679 A targeted DNA vaccine encoding fas ligand defines its dual role in the regulation of experimental autoimmune encephalomyelitis G Wildbaum, J Westermann, G Maor - The Journal of Clinical , 2000 - Am Soc Clin Investighttps://www.jci.org/articles/view/8759

多肽H2N-Tyr-Gly-Ser-Leu-Pro-Gln-Lys-Ser-Gln-Arg-Ser-Gln-Asp-Glu-Asn-Pro-Val-COOH的合成步骤：

1、合成CTC树脂：称取0.79g CTC Resin（如初始取代度约为0.47mmol/g）和0.45mmol Fmoc-Val-OH于反应器中，加入适量DCM溶解氨基酸（需要注意，此时CTC树脂体积会增大好几倍，避免DCM溶液过少），再加入1.11mmol DIPEA（Mw：129.1,d：0.740g/ml），反应2-3小时后，可不抽滤溶液，直接加入1ml的HPLC级甲醇，封端半小时。依次用DMF洗涤2次，甲醇洗涤1次，DCM洗涤一次，甲醇洗涤一次，DCM洗涤一次，DMF洗涤2次（这里使用甲醇和DCM交替洗涤，是为了更好地去除其他溶质，有利于后续反应）。得到  Fmoc-Val-CTC Resin。结构图如下：

2、脱Fmoc：加3倍树脂体积的20%Pip/DMF溶液，鼓氮气30分钟，然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Val-CTC Resin 。（此步骤脱除Fmoc基团，茚三酮检测为蓝色，Pip为哌啶）。结构图如下：

3、缩合：取1.11mmol Fmoc-Pro-OH 氨基酸，加入到上述树脂里，加适当DMF溶解氨基酸，再依次加入2.23mmol DIPEA，1.06mmol HBTU。反应30分钟后，取小样洗涤，茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。得到Fmoc-Pro-Val-CTC Resin。氨基酸：DIPEA：HBTU：树脂=3：6：2.85：1（摩尔比）。结构图如下：

4、依次循环步骤二、步骤三，依次得到

H2N-Pro-Val-CTC Resin

Fmoc-Asn(Trt)-Pro-Val-CTC Resin

H2N-Asn(Trt)-Pro-Val-CTC Resin

Fmoc-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

H2N-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

Fmoc-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

H2N-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

Fmoc-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

H2N-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

Fmoc-Ser(tBu)-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

H2N-Ser(tBu)-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

Fmoc-Arg(Pbf)-Ser(tBu)-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

H2N-Arg(Pbf)-Ser(tBu)-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

Fmoc-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

H2N-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

Fmoc-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

H2N-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

Fmoc-Lys(Boc)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

H2N-Lys(Boc)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

Fmoc-Gln(Trt)-Lys(Boc)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

H2N-Gln(Trt)-Lys(Boc)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

Fmoc-Pro-Gln(Trt)-Lys(Boc)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

H2N-Pro-Gln(Trt)-Lys(Boc)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

Fmoc-Leu-Pro-Gln(Trt)-Lys(Boc)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

H2N-Leu-Pro-Gln(Trt)-Lys(Boc)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

Fmoc-Ser(tBu)-Leu-Pro-Gln(Trt)-Lys(Boc)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

H2N-Ser(tBu)-Leu-Pro-Gln(Trt)-Lys(Boc)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

Fmoc-Gly-Ser(tBu)-Leu-Pro-Gln(Trt)-Lys(Boc)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

H2N-Gly-Ser(tBu)-Leu-Pro-Gln(Trt)-Lys(Boc)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

Fmoc-Tyr(tBu)-Gly-Ser(tBu)-Leu-Pro-Gln(Trt)-Lys(Boc)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin

以上中间结构，均可在专肽生物多肽计算器-多肽结构计算器中，一键画出。

最后再经过步骤二得到 H2N-Tyr(tBu)-Gly-Ser(tBu)-Leu-Pro-Gln(Trt)-Lys(Boc)-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Ser(tBu)-Gln(Trt)-Asp(OtBu)-Glu(OtBu)-Asn(Trt)-Pro-Val-CTC Resin，结构如下：

5、切割：6倍树脂体积的切割液（或每1g树脂加8ml左右的切割液），摇床摇晃 2小时，过滤掉树脂，用冰无水乙醚沉淀滤液，并用冰无水乙醚洗涤沉淀物3次，最后将沉淀物放真空干燥釜中，常温干燥24小试，得到粗品H2N-Tyr-Gly-Ser-Leu-Pro-Gln-Lys-Ser-Gln-Arg-Ser-Gln-Asp-Glu-Asn-Pro-Val-COOH。结构图见产品结构图。

切割液选择：1）TFA：H2O=95%：5%

2）TFA：H2O：TIS=95%：2.5%：2.5%

3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%

（前两种适合没有容易氧化的氨基酸，例如Trp、Cys、Met。第三种适合几乎所有的序列。）

6、纯化冻干：使用液相色谱纯化，收集目标峰液体，进行冻干，获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度 是否目标纯度。

7、最后总结：

杭州专肽生物技术有限公司（ALLPEPTIDE https://www.allpeptide.com）主营定制多肽合成业务，提供各类长肽，短肽，环肽，提供各类修饰肽，如：荧光标记修饰（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基团修饰肽（叠氮、炔基、DBCO、DOTA、NOTA等），同位素标记肽（N15、C13），订书肽（Stapled Peptide）,脂肪酸修饰肽（Pal、Myr、Ste），磷酸化修饰肽（P-Ser、P-Thr、P-Tyr），环肽（酰胺键环肽、一对或者多对二硫键环），生物素标记肽，PEG修饰肽，甲基化修饰肽等。

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