Asp76-pTH（39-84）（人）是一种在生物医学领域广泛使用的合成肽，显示出其作为甲状旁腺激素（pTH）的强效受体拮抗剂的能力，对钙和磷酸盐代谢的复杂动力学产生关键影响。
编号：190770
CAS号：79804-72-1
单字母：H2N-APLAPRDAGSQRPRKKEDNVLVESHEKSLGEADKADVDVLTKAKSQ-OH
| 编号: | 190770 |
| 中文名称: | 甲状旁腺激素 (Asp76)-pTH(39-84)(human) |
| 英文名: | (Asp76)-Parathyroid Hormone(39-84)(human) |
| 英文同义词: | (Asp76)-pTH (39-84) (human) |
| CAS号: | 79804-72-1 |
| 单字母: | H2N-APLAPRDAGSQRPRKKEDNVLVESHEKSLGEADKADVDVLTKAKSQ-OH |
| 三字母: | H2N N端氨基 -Ala丙氨酸 -Pro脯氨酸 -Leu亮氨酸 -Ala丙氨酸 -Pro脯氨酸 -Arg精氨酸 -Asp天冬氨酸 -Ala丙氨酸 -Gly甘氨酸 -Ser丝氨酸 -Gln谷氨酰胺 -Arg精氨酸 -Pro脯氨酸 -Arg精氨酸 -Lys赖氨酸 -Lys赖氨酸 -Glu谷氨酸 -Asp天冬氨酸 -Asn天冬酰胺 -Val缬氨酸 -Leu亮氨酸 -Val缬氨酸 -Glu谷氨酸 -Ser丝氨酸 -His组氨酸 -Glu谷氨酸 -Lys赖氨酸 -Ser丝氨酸 -Leu亮氨酸 -Gly甘氨酸 -Glu谷氨酸 -Ala丙氨酸 -Asp天冬氨酸 -Lys赖氨酸 -Ala丙氨酸 -Asp天冬氨酸 -Val缬氨酸 -Asp天冬氨酸 -Val缬氨酸 -Leu亮氨酸 -Thr苏氨酸 -Lys赖氨酸 -Ala丙氨酸 -Lys赖氨酸 -Ser丝氨酸 -Gln谷氨酰胺 -OHC端羧基 |
| 氨基酸个数: | 46 |
| 分子式: | C211H356N66O73 |
| 平均分子量: | 4985.48 |
| 精确分子量: | 4982.62 |
| 等电点(PI): | 9.28 |
| pH=7.0时的净电荷数: | 1.22 |
| 酸性基团个数: | pH=7.0时的净电荷数：0.11 |
| 碱性基团个数: | 平均亲水性：亲水 |
| 平均亲水性: | 0.94390243902439 |
| 疏水性值: | -1.06 |
| 外观与性状: | 白色粉末状固体 |
| 消光系数: | - |
| 来源: | 人工化学合成，仅限科学研究使用，不得用于人体。 |
| 纯度: | 95%、98% |
| 盐体系: | 可选TFA、HAc、HCl或其它 |
| 储存条件: | 负80℃至负20℃ |
| 标签: | 甲状旁腺(激)素及类似物 拮抗剂相关肽(Antagonist Peptide) |
Asp76-pTH（39-84）（人）是一种在生物医学领域广泛使用的合成肽，显示出其作为甲状旁腺激素（pTH）的强效受体拮抗剂的能力，对钙和磷酸盐代谢的复杂动力学产生关键影响。
Asp76-pTH (39-84) (human) is a synthetic peptide widely utilized in the biomedical sector exhibiting its prowess as a potent receptor antagonist for parathyroid hormone (PTH), exerting pivotal influence over the intricate dynamics of calcium and phosphate metabolism.
| DOI | 名称 | |
|---|---|---|
| 10.1210/endo.143.1.8575 | Human PTH-(7-84) inhibits bone resorption in vitro via actions independent of the type 1 PTH/PTHrP receptor | 下载 |
| 10.1055/s-2007-979139 | The hydrolysis of parathyroid hormone by papain-solubilized rat meprin differs from that of detergent-solubilized meprin | 下载 |
| 10.1083/jcb.135.4.1179 | Differential effects of parathyroid hormone fragments on collagen gene expression in chondrocytes | 下载 |
| 10.1152/ajpendo.1998.274.2.E297 | New insights into interactions between the human PTH/PTHrP receptor and agonist/antagonist binding | 下载 |
多肽H2N-Ala-Pro-Leu-Ala-Pro-Arg-Asp-Ala-Gly-Ser-Gln-Arg-Pro-Arg-Lys-Lys-Glu-Asp-Asn-Val-Leu-Val-Glu-Ser-His-Glu-Lys-Ser-Leu-Gly-Glu-Ala-Asp-Lys-Ala-Asp-Val-Asp-Val-Leu-Thr-Lys-Ala-Lys-Ser-Gln-COOH的合成步骤：
1、合成CTC树脂：称取0.88g CTC Resin（如初始取代度约为0.69mmol/g）和0.73mmol Fmoc-Gln(Trt)-OH于反应器中，加入适量DCM溶解氨基酸（需要注意，此时CTC树脂体积会增大好几倍，避免DCM溶液过少），再加入1.82mmol DIPEA（Mw：129.1,d：0.740g/ml），反应2-3小时后，可不抽滤溶液，直接加入1ml的HPLC级甲醇，封端半小时。依次用DMF洗涤2次，甲醇洗涤1次，DCM洗涤一次，甲醇洗涤一次，DCM洗涤一次，DMF洗涤2次（这里使用甲醇和DCM交替洗涤，是为了更好地去除其他溶质，有利于后续反应）。得到 Fmoc-Gln(Trt)-CTC Resin。结构图如下：
2、脱Fmoc：加3倍树脂体积的20%Pip/DMF溶液，鼓氮气30分钟，然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Gln(Trt)-CTC Resin 。（此步骤脱除Fmoc基团，茚三酮检测为蓝色，Pip为哌啶）。结构图如下：
3、缩合：取1.82mmol Fmoc-Ser(tBu)-OH 氨基酸，加入到上述树脂里，加适当DMF溶解氨基酸，再依次加入3.64mmol DIPEA，1.73mmol HBTU。反应30分钟后，取小样洗涤，茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。得到Fmoc-Ser(tBu)-Gln(Trt)-CTC Resin。氨基酸：DIPEA：HBTU：树脂=3：6：2.85：1（摩尔比）。结构图如下：
4、依次循环步骤二、步骤三，依次得到
H2N-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Gln(Trt)-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Gln(Trt)-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Gly-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Gly-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Ala-Gly-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Ala-Gly-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Asp(OtBu)-Ala-Gly-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Asp(OtBu)-Ala-Gly-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Arg(Pbf)-Asp(OtBu)-Ala-Gly-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Arg(Pbf)-Asp(OtBu)-Ala-Gly-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Pro-Arg(Pbf)-Asp(OtBu)-Ala-Gly-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Pro-Arg(Pbf)-Asp(OtBu)-Ala-Gly-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Ala-Pro-Arg(Pbf)-Asp(OtBu)-Ala-Gly-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Ala-Pro-Arg(Pbf)-Asp(OtBu)-Ala-Gly-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Leu-Ala-Pro-Arg(Pbf)-Asp(OtBu)-Ala-Gly-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Leu-Ala-Pro-Arg(Pbf)-Asp(OtBu)-Ala-Gly-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Pro-Leu-Ala-Pro-Arg(Pbf)-Asp(OtBu)-Ala-Gly-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
H2N-Pro-Leu-Ala-Pro-Arg(Pbf)-Asp(OtBu)-Ala-Gly-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
Fmoc-Ala-Pro-Leu-Ala-Pro-Arg(Pbf)-Asp(OtBu)-Ala-Gly-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin
以上中间结构，均可在专肽生物多肽计算器-多肽结构计算器中，一键画出。
最后再经过步骤二得到 H2N-Ala-Pro-Leu-Ala-Pro-Arg(Pbf)-Asp(OtBu)-Ala-Gly-Ser(tBu)-Gln(Trt)-Arg(Pbf)-Pro-Arg(Pbf)-Lys(Boc)-Lys(Boc)-Glu(OtBu)-Asp(OtBu)-Asn(Trt)-Val-Leu-Val-Glu(OtBu)-Ser(tBu)-His(Trt)-Glu(OtBu)-Lys(Boc)-Ser(tBu)-Leu-Gly-Glu(OtBu)-Ala-Asp(OtBu)-Lys(Boc)-Ala-Asp(OtBu)-Val-Asp(OtBu)-Val-Leu-Thr(tBu)-Lys(Boc)-Ala-Lys(Boc)-Ser(tBu)-Gln(Trt)-CTC Resin，结构如下：
5、切割：6倍树脂体积的切割液（或每1g树脂加8ml左右的切割液），摇床摇晃 2小时，过滤掉树脂，用冰无水乙醚沉淀滤液，并用冰无水乙醚洗涤沉淀物3次，最后将沉淀物放真空干燥釜中，常温干燥24小试，得到粗品H2N-Ala-Pro-Leu-Ala-Pro-Arg-Asp-Ala-Gly-Ser-Gln-Arg-Pro-Arg-Lys-Lys-Glu-Asp-Asn-Val-Leu-Val-Glu-Ser-His-Glu-Lys-Ser-Leu-Gly-Glu-Ala-Asp-Lys-Ala-Asp-Val-Asp-Val-Leu-Thr-Lys-Ala-Lys-Ser-Gln-COOH。结构图见产品结构图。
切割液选择：1）TFA：H2O=95%：5%
2）TFA：H2O：TIS=95%：2.5%：2.5%
3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%
（前两种适合没有容易氧化的氨基酸，例如Trp、Cys、Met。第三种适合几乎所有的序列。）
6、纯化冻干：使用液相色谱纯化，收集目标峰液体，进行冻干，获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度 是否目标纯度。
7、最后总结：
杭州专肽生物技术有限公司（ALLPEPTIDE https://www.allpeptide.com）主营定制多肽合成业务，提供各类长肽，短肽，环肽，提供各类修饰肽，如：荧光标记修饰（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基团修饰肽（叠氮、炔基、DBCO、DOTA、NOTA等），同位素标记肽（N15、C13），订书肽（Stapled Peptide）,脂肪酸修饰肽（Pal、Myr、Ste），磷酸化修饰肽（P-Ser、P-Thr、P-Tyr），环肽（酰胺键环肽、一对或者多对二硫键环），生物素标记肽，PEG修饰肽，甲基化修饰肽等。
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