一种有效的、选择性的 ComD1 受体激动剂，IC50 值为 10.3 nM。CSP1 是能力刺激肽 (CSP) 的主要变体，它可以通过调节群体感应 (QS) 来调节肺炎链球菌的遗传转化。
编号：190836
CAS号：172889-49-5
单字母：H2N-EMRLSKFFRDFILQRKK-OH
| 编号: | 190836 |
| 中文名称: | CSP |
| 英文名: | CSP |
| CAS号: | 172889-49-5 |
| 单字母: | H2N-EMRLSKFFRDFILQRKK-OH |
| 三字母: | H2N N端氨基 -Glu谷氨酸 -Met甲硫氨酸 -Arg精氨酸 -Leu亮氨酸 -Ser丝氨酸 -Lys赖氨酸 -Phe苯丙氨酸 -Phe苯丙氨酸 -Arg精氨酸 -Asp天冬氨酸 -Phe苯丙氨酸 -Ile异亮氨酸 -Leu亮氨酸 -Gln谷氨酰胺 -Arg精氨酸 -Lys赖氨酸 -Lys赖氨酸 -OHC端羧基 |
| 氨基酸个数: | 17 |
| 分子式: | C103H168N30O24S1 |
| 平均分子量: | 2242.69 |
| 精确分子量: | 2241.26 |
| 等电点(PI): | 12.27 |
| pH=7.0时的净电荷数: | 4.98 |
| 平均亲水性: | 0.60588235294118 |
| 疏水性值: | -0.83 |
| 外观与性状: | 白色粉末状固体 |
| 消光系数: | - |
| 来源: | 人工化学合成，仅限科学研究使用，不得用于人体。 |
| 纯度: | 95%、98% |
| 盐体系: | 可选TFA、HAc、HCl或其它 |
| 储存条件: | 负80℃至负20℃ |
| 标签: | 激动剂多肽(Agonist Peptide) 细菌肽(Bacterial Peptides) |
CSP1 是一种有效的、选择性的 ComD1 受体激动剂，IC50 值为 10.3 nM。CSP1 是能力刺激肽 (CSP) 的主要变体，它可以通过调节群体感应 (QS) 来调节肺炎链球菌的遗传转化。CSP1 可以作为抗菌剂。
CSP1 is a potent and selective ComD1 receptor agonist, with an IC50 of 10.3 nM. CSP1 is a major variants of competence-stimulating peptide (CSP), and it can regulate genetic transformation of S. pneumonia by modulating quorum sensing (QS). CSP1 can act as an antibacterial agent.
CSP-1，能力刺激肽-1是肺炎链球菌分泌的一种17个氨基酸的信息素。CSP信息素在体内用于细胞间通讯。该肽激活信号转导通路ComABCDE，调节自然遗传转化。信息素是核糖体合成的前体肽。成熟的信息素是菌株特异性的。CSP信息素由肺炎链球菌Rx菌株产生，与R6菌株关系密切。
CSP-1, competence stimulating peptide-1 is a 17 amino acids (EMRLSKFFRDFILQRKK) pheromone that is secreted by Streptococcus pneumoniae. CSP pheromone is used in-vivo for intercellular communication. This peptide activates signal transduction pathway ComABCDE, which regulates natural genetic transformation. The pheromone is ribosomally synthesized as precursor peptide. The mature pheromone is strain specific. CSP pheromone is produced by S. pneumoniae strain Rx, which is closely related to strain R6.
Definition
Bacterial peptides are protein fragments which are either part of a bacterium or produced by a bacteria1.
Classification
Different classes of peptides are produced by bacteria. Some examples include, antibiotics, enterotoxins, flagellar proteins, lipoproteins and various enzymes1.
Structural Characteristics
Structural characteristics of some bacterial peptides are described below-
A) Malaria merozoite surface peptide (MSP-1): It is synthesized as a large precursor on the surface of the bacterium Plasmodium falciparum. Proteolytic cleavage results in the production of a 19 KDa product whose tertiary structure is maintained by disulphide bridges2.
B) Giardia variable surface protein: This peptide is the specific conserved region of the Giardia variable surface proteins (VSPs) that are cysteine rich zinc finger proteins. VSPs differ in size and sequence, they are characterized by this highly conserved C-terminal membrane spanning region, a hydrophilic cytoplasmic tail with a conserved five amino acid CRGKA signature sequence3,4.
C) P.falciparum liver stage antigen 3: The protein is 200Kda and is highly conserved among parasites from different geographic regions5.
Mode of action
A) MSP-1 is known to trigger antibody response by CD4 helper T cells. It is likely that these cells bind to the C-terminal domain of MSP-12.
B) VSPs have a conserved hydrophilic amono acid trail that is palmitoyted by palmityl tranferases upon which they are activated3,4.
C) P. falciparum liver stage antigen 3 is a potent antigen that is recongnized by T cells5.
Functions
A) MSP-1 is a vaccine candidate for Plasmodium falciparum infection. It triggers a CD-4 T cell response2.
B) VSPs are necessary for survival in the environment and host infection3,4.
C) P.falciparum stage antigen 3 is also a good candidate vaccine as it activates both T and B cell responses5.
References
1. Gitai Z (2005). "The new bacterial cell biology: moving parts and subcellular architecture". Cell, 120 (5): 577–86.
2. Stuart JQ and Jean L (2001). Different regions of the malaria merozoite surface protein 1 of Plasmodium chabaudi elicit distinct T-cell and antibody isotype responses. Infect Immun, 69(4): 2245–2251.
3. Davids BJ, Reiner DS, Birkeland SR, Preheim SP, Cipriano MJ, McArthur AG, Gillin FD (2006). A New Family of Giardial Cysteine-Rich Non-VSP Protein Genes and a Novel Cyst Protein. PLoS ONE, 20,1:e44.
4. Touz MC, Conrad JT, Nash TE (2005). A novel palmitoyl acyl transferase controls surface protein palmitoylation and cytotoxicity in Giardia lamblia. Mol Microbiol., 58 (4), 999-1011.
5. Jean-Pierre S, Blanca LP, Karima B, Pierra D, Pierra D (2001). DNA Immunization by Plasmodium falciparum liver-stage antigen 3 induces protection against Plasmodium yoelii Sporozoite challenge. Infect Immun., 69, 1202–1206.
Yang Y, et, al. Structural Characterization of Competence-Stimulating Peptide Analogues Reveals Key Features for ComD1 and ComD2 Receptor Binding in Streptococcus pneumonia. Biochemistry. 2018 Sep 11;57(36):5359-5369. : https://pubmed.ncbi.nlm.nih.gov/30125091/
Johnsborg O, et, al. A hydrophobic patch in the competence-stimulating Peptide, a pneumococcal competence pheromone, is essential for specificity and biological activity. J Bacteriol. 2006 Mar; 188(5): 1744-9. : https://pubmed.ncbi.nlm.nih.gov/16484185/
