肽 Lv 是通过基于计算生物信息学的筛选过程发现的。神经肽在视网膜感光层、海马、嗅球、小脑、大脑皮层、肺、脾、肝和肠中表达。肽 Lv 增强了视网膜光感受器中的 L 型电压门控钙通道 (L-VGCC) 电流。
编号：200292
CAS号：1872441-58-1
单字母：H2N-DSLLAVRWFFAPDGSQEALMVKMTKLRVIQYYGNFSRIANQQRLRLLEE-OH
| 编号: | 200292 |
| 中文名称: | Peptide Lv (rat) |
| CAS号: | 1872441-58-1 |
| 单字母: | H2N-DSLLAVRWFFAPDGSQEALMVKMTKLRVIQYYGNFSRIANQQRLRLLEE-OH |
| 三字母: | H2N N端氨基 -Asp天冬氨酸 -Ser丝氨酸 -Leu亮氨酸 -Leu亮氨酸 -Ala丙氨酸 -Val缬氨酸 -Arg精氨酸 -Trp色氨酸 -Phe苯丙氨酸 -Phe苯丙氨酸 -Ala丙氨酸 -Pro脯氨酸 -Asp天冬氨酸 -Gly甘氨酸 -Ser丝氨酸 -Gln谷氨酰胺 -Glu谷氨酸 -Ala丙氨酸 -Leu亮氨酸 -Met甲硫氨酸 -Val缬氨酸 -Lys赖氨酸 -Met甲硫氨酸 -Thr苏氨酸 -Lys赖氨酸 -Leu亮氨酸 -Arg精氨酸 -Val缬氨酸 -Ile异亮氨酸 -Gln谷氨酰胺 -Tyr酪氨酸 -Tyr酪氨酸 -Gly甘氨酸 -Asn天冬酰胺 -Phe苯丙氨酸 -Ser丝氨酸 -Arg精氨酸 -Ile异亮氨酸 -Ala丙氨酸 -Asn天冬酰胺 -Gln谷氨酰胺 -Gln谷氨酰胺 -Arg精氨酸 -Leu亮氨酸 -Arg精氨酸 -Leu亮氨酸 -Leu亮氨酸 -Glu谷氨酸 -Glu谷氨酸 -OHC端羧基 |
| 氨基酸个数: | 49 |
| 分子式: | C262H415N73O72S2 |
| 平均分子量: | 5803.67 |
| 精确分子量: | 5800.05 |
| 等电点(PI): | 11.48 |
| pH=7.0时的净电荷数: | 4.98 |
| 平均亲水性: | -0.11521739130435 |
| 疏水性值: | -0.19 |
| 消光系数: | 8480 |
| 来源: | 人工化学合成，仅限科学研究使用，不得用于人体。 |
| 储存条件: | 负80℃至负20℃ |
| 标签: | 离子通道(Ion channels)相关肽 |
Peptide Lv has been discovered via a computational bioinformatics-based screening process. The neuropeptide is expressed in retinal photoreceptor layer, hippocampus, olfactory bulb, cerebellum, cerebral cortex, lung, spleen, liver and intestine. Peptide Lv enhances L-type voltage-gated calcium channel (L-VGCC) currents in retinal photoreceptors.
Definition
Ion channels are membrane protein complexes and their function is to facilitate the diffusion of ions across biological membranes.
Discovery
British biophysicists Alan Hodgkin and Andrew Huxley hypothesized the existence of ion channels as a part of their Nobel Prize-winning theory of the nerve impulse, published in 1952. Existence of channel was confirmed in the 1970’s with an electrical recording technique known as the "patch clamp," by Erwin Neher and Bert Sakmann, the technique's inventors 1. 2003 Nobel Laureates Peter Agre and Roderick MacKinnon, have analysed the physico-chemical properties of ion channel functions, all of which are needed for the cell to function .
Structural Characteristics
In 1990s the first structure of an ion channel solved at atomic resolution <0.3nm is that of the bacterial porins, a family of homo-trimeric channel proteins. Each subunit contains 16 to 18 transmembrane, anti-parallel beta-strands forming a beta-barrel structure. The beta-strands are amphipathic, they contain alternating polar and non-polar residues and the inter-strand interaction is fully saturated with H-bonding. This creates a hydrophilic pore interior providing a water filled channel. The channel has a large diameter of 0.8x1.1nm, and is non-selective for small ions. However, it has an upper exclusion size limit corresponding to molecular weights of about .6 K Da. Most metabolites have molecular weights lower than .6 KDa and have been shown to pass through porin channels .
Mode of Action
An ion channel is an integral membrane protein or more typically an assembly of several proteins. Such "multi-subunit" assemblies usually involve a circular arrangement of identical or related proteins closely packed around a water-filled pore through the plane of the membrane or lipid bilayer. While large-pore channels permit the passage of ions more or less indiscriminately, the archetypal channel pore is just one or two atoms wide at its narrowest point, it conducts a specific species of ion, such as sodium or potassium, and conveys them through the membrane single file--nearly as fast as the ions move through free fluid. Access to the pore is governed by "gates," which may be opened or closed by chemical or electrical signals, or mechanical force, depending on the variety of channel. There are different groups of channels,
- Ligand gated channels neurotransmitters
- Voltage gated channels transmembrane potential (electric field)
- Second messenger gated channels nucleotides, G-proteins
- Mechanosensitive channels osmotic pressure, membrane curvature
- Gap junctions, porins not gated
X-ray analysis potassium channel from Streptomyces lividans(called K1) reveals that four identical subunits create an inverted teepee, or cone, cradling the selectivity filter of the pore in its outer end. The narrow selectivity filter is only 12? long, whereas the remainder of the pore is wider and lined with hydrophobic amino acids. A large water-filled cavity and helix dipoles are positioned so as to overcome electrostatic destabilization of an ion in the pore at the center of the bilayer. Main chain carbonyl oxygen atoms from the K1 channel signature sequence line the selectivity filter, which is held open by structural constraints to coordinate K1 ions but not smaller Na1 ions. The selectivity filter contains two K1 ions about 7.5 angstroms apart. This configuration promotes ion conduction by exploiting electrostatic repulsive forces to overcome attractive forces between K1 ions and the selectivity filter. The architecture of the pore establishes the physical principles underlying selective K1 conduction 2.
Functions
Role in nervous systems, channels are especially prominent components of the nervous system, "voltage-gated" channels conduct the nerve impulse and "transmitter-gated" channels mediate conduction across the synapses. Many toxins usually act on channels for shutting down the nervous systems of predators and prey 3.
Biological role, ion channels figure in a wide variety of biological processes that involve rapid changes in cells, such as cardiac, skeletal, and smooth muscle contraction, epithelial transport of nutrients and ions, T-cell activation and pancreatic beta-cell insulin release 4.
Drug targets, ion channels are the main targets of many drugs already used in the clinics. Most of these drugs were introduced in therapy based on the experience acquired quite empirically, and many were discovered afterward to target ion channels. Intense research is being conducted to develop new drugs acting selectively onion channel subtypes and aimed at the understanding of the intimate drug–channel interaction. Polymorphisms or mutations in ion channel genes modify sensitivity to drugs, opening the way toward the development of pharmacogenetics 5.
References
Book: Neuroscience, Ion Channels Underlying Action Potentials., by Dale P, George A, Sunderland (MA): Sinauer Associates, Inc.; 2001
2. Doyle DA, Morais CJ, Pfuetzner RA, Kuo A, Gulbis JM, Cohen SL, Chait BT, MacKinnon R (1998). The structure of the potassium channel: molecular basis of K+ conduction and selectivity. Science, 280(5360):69-77.
3. Camerino DC, Tricarico D, Desaphy JF (2007). Ion channel pharmacology. Neurotherapeutics, 4(2):184-98.
4. Book: Chapter 6: Electrical Excitability and Ion Channels.by Basic neurochemistry: molecular, cellular, and medical aspects., by Hille B, Catterall WA. Philadelphia: Lippincott-Raven.
5. Camerino DC, Desaphy JF, Tricarico D, Pierno S, Liantonio A (2008). Therapeutic approaches to ion channel diseases. Adv. Genet., 64:81-145.
| DOI | 名称 | |
|---|---|---|
| 10.1371/journal.pone.0043091 | Identification of Peptide lv, a novel putative neuropeptide that regulates the expression of L-type voltage-gated calcium channels in photoreceptors | 下载 |
多肽H2N-Asp-Ser-Leu-Leu-Ala-Val-Arg-Trp-Phe-Phe-Ala-Pro-Asp-Gly-Ser-Gln-Glu-Ala-Leu-Met-Val-Lys-Met-Thr-Lys-Leu-Arg-Val-Ile-Gln-Tyr-Tyr-Gly-Asn-Phe-Ser-Arg-Ile-Ala-Asn-Gln-Gln-Arg-Leu-Arg-Leu-Leu-Glu-Glu-COOH的合成步骤：
1、合成CTC树脂：称取1.54g CTC Resin（如初始取代度约为0.88mmol/g）和1.63mmol Fmoc-Glu(OtBu)-OH于反应器中，加入适量DCM溶解氨基酸（需要注意，此时CTC树脂体积会增大好几倍，避免DCM溶液过少），再加入4.07mmol DIPEA（Mw：129.1,d：0.740g/ml），反应2-3小时后，可不抽滤溶液，直接加入1ml的HPLC级甲醇，封端半小时。依次用DMF洗涤2次，甲醇洗涤1次，DCM洗涤一次，甲醇洗涤一次，DCM洗涤一次，DMF洗涤2次（这里使用甲醇和DCM交替洗涤，是为了更好地去除其他溶质，有利于后续反应）。得到 Fmoc-Glu(OtBu)-CTC Resin。结构图如下：
2、脱Fmoc：加3倍树脂体积的20%Pip/DMF溶液，鼓氮气30分钟，然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Glu(OtBu)-CTC Resin 。（此步骤脱除Fmoc基团，茚三酮检测为蓝色，Pip为哌啶）。结构图如下：
3、缩合：取4.07mmol Fmoc-Glu(OtBu)-OH 氨基酸，加入到上述树脂里，加适当DMF溶解氨基酸，再依次加入8.13mmol DIPEA，3.86mmol HBTU。反应30分钟后，取小样洗涤，茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。得到Fmoc-Glu(OtBu)-Glu(OtBu)-CTC Resin。氨基酸：DIPEA：HBTU：树脂=3：6：2.85：1（摩尔比）。结构图如下：
4、依次循环步骤二、步骤三，依次得到
H2N-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Ala-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Ala-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Phe-Ala-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Phe-Ala-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Phe-Phe-Ala-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Phe-Phe-Ala-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Trp(Boc)-Phe-Phe-Ala-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Trp(Boc)-Phe-Phe-Ala-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Arg(Pbf)-Trp(Boc)-Phe-Phe-Ala-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Arg(Pbf)-Trp(Boc)-Phe-Phe-Ala-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Val-Arg(Pbf)-Trp(Boc)-Phe-Phe-Ala-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Val-Arg(Pbf)-Trp(Boc)-Phe-Phe-Ala-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Ala-Val-Arg(Pbf)-Trp(Boc)-Phe-Phe-Ala-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Ala-Val-Arg(Pbf)-Trp(Boc)-Phe-Phe-Ala-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Leu-Ala-Val-Arg(Pbf)-Trp(Boc)-Phe-Phe-Ala-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Leu-Ala-Val-Arg(Pbf)-Trp(Boc)-Phe-Phe-Ala-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Leu-Leu-Ala-Val-Arg(Pbf)-Trp(Boc)-Phe-Phe-Ala-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Leu-Leu-Ala-Val-Arg(Pbf)-Trp(Boc)-Phe-Phe-Ala-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Ser(tBu)-Leu-Leu-Ala-Val-Arg(Pbf)-Trp(Boc)-Phe-Phe-Ala-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
H2N-Ser(tBu)-Leu-Leu-Ala-Val-Arg(Pbf)-Trp(Boc)-Phe-Phe-Ala-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
Fmoc-Asp(OtBu)-Ser(tBu)-Leu-Leu-Ala-Val-Arg(Pbf)-Trp(Boc)-Phe-Phe-Ala-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin
以上中间结构，均可在专肽生物多肽计算器-多肽结构计算器中，一键画出。
最后再经过步骤二得到 H2N-Asp(OtBu)-Ser(tBu)-Leu-Leu-Ala-Val-Arg(Pbf)-Trp(Boc)-Phe-Phe-Ala-Pro-Asp(OtBu)-Gly-Ser(tBu)-Gln(Trt)-Glu(OtBu)-Ala-Leu-Met-Val-Lys(Boc)-Met-Thr(tBu)-Lys(Boc)-Leu-Arg(Pbf)-Val-Ile-Gln(Trt)-Tyr(tBu)-Tyr(tBu)-Gly-Asn(Trt)-Phe-Ser(tBu)-Arg(Pbf)-Ile-Ala-Asn(Trt)-Gln(Trt)-Gln(Trt)-Arg(Pbf)-Leu-Arg(Pbf)-Leu-Leu-Glu(OtBu)-Glu(OtBu)-CTC Resin，结构如下：
5、切割：6倍树脂体积的切割液（或每1g树脂加8ml左右的切割液），摇床摇晃 2小时，过滤掉树脂，用冰无水乙醚沉淀滤液，并用冰无水乙醚洗涤沉淀物3次，最后将沉淀物放真空干燥釜中，常温干燥24小试，得到粗品H2N-Asp-Ser-Leu-Leu-Ala-Val-Arg-Trp-Phe-Phe-Ala-Pro-Asp-Gly-Ser-Gln-Glu-Ala-Leu-Met-Val-Lys-Met-Thr-Lys-Leu-Arg-Val-Ile-Gln-Tyr-Tyr-Gly-Asn-Phe-Ser-Arg-Ile-Ala-Asn-Gln-Gln-Arg-Leu-Arg-Leu-Leu-Glu-Glu-COOH。结构图见产品结构图。
切割液选择：1）TFA：H2O=95%：5%
2）TFA：H2O：TIS=95%：2.5%：2.5%
3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%
（前两种适合没有容易氧化的氨基酸，例如Trp、Cys、Met。第三种适合几乎所有的序列。）
6、纯化冻干：使用液相色谱纯化，收集目标峰液体，进行冻干，获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度 是否目标纯度。
7、最后总结：
杭州专肽生物技术有限公司（ALLPEPTIDE https://www.allpeptide.com）主营定制多肽合成业务，提供各类长肽，短肽，环肽，提供各类修饰肽，如：荧光标记修饰（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基团修饰肽（叠氮、炔基、DBCO、DOTA、NOTA等），同位素标记肽（N15、C13），订书肽（Stapled Peptide）,脂肪酸修饰肽（Pal、Myr、Ste），磷酸化修饰肽（P-Ser、P-Thr、P-Tyr），环肽（酰胺键环肽、一对或者多对二硫键环），生物素标记肽，PEG修饰肽，甲基化修饰肽等。
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