| 编号: | 434441 |
| 中文名称: | CEF5, Influenza Virus NP (91 - 99) |
| 英文名: | CEF5, Influenza Virus NP (91 - 99) |
| 单字母: | H2N-KTGGPIYKR-OH |
| 三字母: | H2N N端氨基 -Lys赖氨酸 -Thr苏氨酸 -Gly甘氨酸 -Gly甘氨酸 -Pro脯氨酸 -Ile异亮氨酸 -Tyr酪氨酸 -Lys赖氨酸 -Arg精氨酸 -OHC端羧基 |
| 氨基酸个数: | 9 |
| 分子式: | C46H78N14O12 |
| 平均分子量: | 1019.2 |
| 精确分子量: | 1018.59 |
| 等电点(PI): | - |
| pH=7.0时的净电荷数: | 4.97 |
| 平均亲水性: | 0.56666666666667 |
| 疏水性值: | -1.31 |
| 消光系数: | 1490 |
| 标签: | CEF Control Peptides |
Definition
The CEF control peptides are 8-12 amino acids in length, with sequences derived from the human Cytomegalovirus, Epstein-Barr Virus and Influenza Virus1 These peptides are used in the stimulation of IFNg release from CD8+ T cells in individuals with defined HLA types1. They are useful as positive control peptides in several cytokine assays such as Elispot.
Discovery
CEF peptides were first selected in 2002 based on their ability to recognize CD8+ T cells1.
Classification
They are derived from epitopes of viruses and hence have antigenic properties1.
Structural Characteristics
CEF peptides are 8-11 amino acids long with sequences: GILGFVFTL (Influenza A, HLA-A2), FMYSDFHFI (Influenza A, HLA-A2), CLGGLLTMV (EBV, HLA-A2), GLCTLVAML (EBV, HLA-A2), NLVPMVATV (HCMV, HLA-A2).
Mode of action
CEF peptides are effective epitopes for CD8+ T cells2. They bind to these cells and trigger the production of IFNg.
Functions
CEF control peptides are used as positive control in Elispot assay that is used to investigate specific immune responses in various diseases including infections, cancer, allergies and autoimmune diseases2. In this case the CEF peptides ensure that the cells under study are active and viable2. Elispot is also useful in the development of vaccines especially for HIV where CEF peptides are used also as controls2.
References
1. Currier JR, Kuta EG, Turk E, Earhart LB, Loomis-Price L, Janetzki S, Ferrari G, Birx DL, Cox JH (2002). A panel of MHC class I restricted viral peptides for use as a quality control for vaccine trial ELISPOT assays, J Immunol Methods, 260, 157-172.
2. Gazagne A, Claret E, Wijdenes J, Yssel H, Bousquet F, Levy E, Vielh P, Scotte F, Goupil T, Fridman WH, Tartour E (2003). A Fluorospot assay to detect single T lymphocytes simultaneously producing multiple cytokines, J Immunol Methods, 283(1-2), 91-98.
